| Full text | |
| Author(s): |
Ogias, Daniela
[1]
;
Rattes, Isadora C.
[1]
;
Hosoya, Larissa Y. M.
[1]
;
Zulian, Juliana G.
[1]
;
Yan, Chao Yun Irene
[1]
;
Gama, Patricia
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 8, JUN 29 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Neonatal-Maternal Separation (NMS) deprives mammals from breastfeeding and maternal care, influencing growth during suckling-weaning transition. In the gastric mucosa, Mist1 (encoded by Bhlha15 gene) and moesin organize the secretory apparatus for pepsinogen C in zymogenic cells. Our current hypothesis was that NMS would change corticosterone activity through receptors (GR), which would modify molecules involved in zymogenic cell differentiation in rats. We found that NMS increased corticosterone levels from 18 days onwards, as GR decreased in the gastric mucosa. However, as nuclear GR was detected, we investigated receptor binding to responsive elements (GRE) and observed an augment in NMS groups. Next, we demonstrated that NMS increased zymogenic population (18 and and 30 days), and targeted Mist1 and moesin. Finally, we searched for evolutionarily conserved sequences that contained GRE in genes involved in pepsinogen C secretion, and found that the genomic regions of Bhlha15 and PgC contained sites highly likely to be responsive to glucocorticoids. We suggest that NMS triggers GR-GRE to enhance the expression and to prime genes that organize cellular architecture in zymogenic population for PgC function. As pepsinogen C-pepsin is essential for digestion, disturbance of parenting through NMS might alter functions of gastric mucosa in a permanent manner. (AU) | |
| FAPESP's process: | 12/10948-9 - Neonatal maternal separation impacts on gastric mucosa development in pups and adult rats: Role of corticosterone and GR |
| Grantee: | Daniela Ogias |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/17415-3 - Postnatal development of gastric mucosa: interaction among feeding, hormones and growth factors |
| Grantee: | Patrícia Gama |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/21449-9 - Gene expression, cell differentiation and gastric functions: can alimentary changes during postnatal development disturb the growth program in rats? |
| Grantee: | Patrícia Gama |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/03076-0 - Cell differentiation in the gastric epithelium of rats: evaluation of corticosterone action |
| Grantee: | Juliana Guimarães Zulian |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/18459-0 - Cell differentiation in the gastric mucosa of rats: role of dietary patterns during postnatal development |
| Grantee: | Larissa Yukari Massarenti Hosoya |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |