Economic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systems

Autor(es)

Torres-Acosta, Mario A. ^{[1, 2]} ; Pereira, Jorge F. B. ^[3] ; Freire, Mara G. ^[4] ; Aguilar-Yanez, Jose M. ^[1] ; Coutinho, Joao A. P. ^[4] ; Titchener-Hooker, Nigel J. ^[2] ; Rito-Palomares, Marco ^[5]

Afiliação do(s) autor(es)

^[1] Escuela Ingn & Ciencias, Tecnol Monterrey, Ave Eugenio Garza Sada 2501 Sur, Monterrey 64849, NL - Mexico

^[2] UCL, Dept Biochem Engn, Adv Ctr Biochem Engn, Torrington Pl, London WC1E 7JE - England

^[3] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Rodovia Araraquara Jau Km 01, BR-14801902 Araraquara, SP - Brazil

^[4] Univ Aveiro, Chem Dept, CICECO Aveiro Inst Mat, Campus Univ Santiago, P-3810193 Aveiro - Portugal

^[5] Escuela Med & Ciencias Salud, Tecnol Monterrey, Ave Morons Prieto 3000 Pte, Monterrey 64710, NL - Mexico

• Tipo de documento: Artigo Científico
• Fonte: Separation and Purification Technology; v. 203, p. 178-184, SEP 12 2018.
• Citações Web of Science: 4

Resumo

Antibiotics are a key pharmaceutical to inhibit growth or kill microorganisms. They represent a profitable market and, in particular, tetracycline has been listed as an essential medicine by the WHO. Therefore it is important to improve their production processes. Recently novel and traditional aqueous two-phase systems for the extraction have been developed with positive results. The present work performs an economic analysis of the production and recovery of tetracycline through the use of several ATPS through bioprocess modeling using specialized software (BioSolve, Biopharm Services Ltd, UK) to determine production costs per gram (CoG/g). First, a virtual model was constructed using published data on the recovery of tetracycline and extended to incorporate uncertainties. To determine how the model behaved, a sensitivity analysis and Monte Carlo simulations were performed. Results showed that ATPS formed by cholinium chloride/K3PO4 was the best option to recover tetracycline, as it had the lowest CoG/g (US\$ 672.83/g), offered the highest recovery yield (92.42%), second best sample input capacity (45% of the ATPS composition) and one of the lowest materials contribution to cost. The ionic liquid-based method of ATPS is a promising alternative for recovering tetracycline from fermentation broth. (AU)

• Processo FAPESP: 14/16424-7 - Otimização e aumento de escala do processo de extração líquido-líquido com líquidos iônicos (LIs) como ferramenta de separação sustentável do biofármaco antileucêmico L-asparaginase (ASPase)
• Beneficiário: Jorge Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores