Otimização e aumento de escala do processo de extração líquido-líquido com líquidos iônicos (LIs) como ferramenta de separação sustentável do biofármaco antileucêmico L-asparaginase (ASPase)

• Processo: 14/16424-7
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Data de Início da vigência: 01 de fevereiro de 2015
• Data de Término da vigência: 31 de janeiro de 2020
• Área do conhecimento: Engenharias - Engenharia Química - Tecnologia Química
• Pesquisador responsável: Jorge Pereira
• Beneficiário: Jorge Pereira
• Instituição Sede: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brasil
• Pesquisadores associados: Adalberto Pessoa Junior ; Sandro Roberto Valentini ; Sónia Patrícia Marques Ventura ; Valéria de Carvalho Santos Ebinuma
• Auxílio(s) vinculado(s): 18/50009-8 - Understanding the molecular interactions between ionic liquids and biopharmaceuticals: the key for a proper design of downstream processing, AP.R SPRINT; 15/50058-0 - Characterization and modelling of aqueous two-phase systems (ATPS) composed of ionic liquids (ILs) and polymers: a boost towards develiping sustainable biopharmaceutical separion, AP.R SPRINT
• Bolsa(s) vinculada(s): 16/09606-7 - Estudo de estabilidade e atividade do anti-leucêmico L-asparaginase na presença de diferentes líquidos iônicos e polímeros, BP.IC; 16/06107-0 - Estudo de novos sistemas de duas fases aquosas combinando líquidos iônicos e polietilenoglicol mono- e di-substituído, BP.IC
• Assunto(s): Extração líquido-líquido Líquidos iônicos Asparaginase Fármacos Sistema de duas fases aquosas
• Palavra(s)-Chave do Pesquisador: biofármacos | Extração Líquido-Líquido | L-asparaginase | líquidos iônicos | purificação | Sistemas Duas Fases Aquosas | Engenharia de Bioprocessos

Resumo

O desenvolvimento de novos biofármacos para serem utilizados em novas terapias é uma das maiores preocupações da Indústria Farmacêutica, especialmente com a emergência de novas resistências aos fármacos convencionais. Adicionalmente, o crescimento exponencial das indústrias de genéricos no mercado, obrigou a Indústria Farmacêutica a diminuir os seus custos operacionais, relacionados maioritariamente com os processos de produção e purificação. O presente projeto de pesquisa propõe a utilização de uma metodologia alternativa e mais sustentável quando comparada com os processos convencionais para a purificação de biofármacos a partir de meios fermentados. Assim, pretende-se purificar e isolar a partir de meios fermentados o anti-leucêmico L-Asparaginase (ASPase) por aplicação de sistemas de duas fases aquosas (SDFA) com líquidos iônicos (LIs), como agentes de formação de fases (polímero-LI, ou polímero-sal-LI). Numa primeira fase, serão caracterizados diversos SDFA pela aplicação de diferentes LIs, objetivando a determinação e caracterização termodinâmica dos vários dos SDFA-LIs, bem como desenvolvimento de modelos para simular e prever sua formação. Numa segunda fase, será estudada a atividade, estabilidade e otimizada a extração da ASPase comercial em escala laboratorial. Na terceira fase será avaliada e otimizada a purificação do biofármaco obtido a partir do cultivo submerso de Pichia pastoris recombinante, sendo esse purificado num modelo à escala piloto durante a última etapa do projeto. Visando uma futura possível aplicação industrial, o aumento de escala dos SDFA será realizado para processos em batelada e em contínuo. Com este projeto pretende-se desenvolver e efetivar a aplicabilidade de novas metodologias que permitam a purificação de biofármacos com elevado interesse farmacêutico, industrial e econômico, através de processos mais sustentáveis, e com maiores índices de seletividade e rendimentos de purificação. (AU)

Publicações científicas

(45)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

DOS SANTOS, NATHALIA VIEIRA; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; BRANDAO PEREIRA, JORGE FERNANDO. Liquid-liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, SI, p. 1845-1863, JUL 2018. (14/16424-7, 14/01580-3, 14/19793-3, 13/08617-7, 16/07529-5)

MARTINS, MARGARIDA; OOI, CHIEN WEI; NEVES, MARCIA C.; PEREIRA, JORGE F. B.; COUTINHO, JOAO A. P.; VENTURA, SONIA P. M.. Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, SI, p. 1864-1870, JUL 2018. (14/16424-7)

MAGRI, AGNES; SOLER, MATHEUS F.; LOPES, ANDRE M.; CILLI, EDUARDO M.; BARBER, PATRICK S.; PESSOA, JR., ADALBERTO; PEREIRA, JORGE F. B.. A critical analysis of L-asparaginase activity quantification methods-colorimetric methods versus high-performance liquid chromatography. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 410, n. 27, p. 6985-6990, NOV 2018. (14/16424-7)

TORRES-ACOSTA, MARIO A.; DOS SANTOS, V, NATHALIA; VENTURA, SONIA P. M.; COUTINHO, JOAO A. P.; RITO-PALOMARES, MARCO; PEREIRA, JORGE F. B.. Economic analysis of the production and recovery of green fluorescent protein using ATPS-based bioprocesses. Separation and Purification Technology, v. 254, JAN 1 2021. (14/16424-7, 14/19793-3, 16/07529-5)

DOS SANTOS, NATHALIA VIEIRA; SAPONI, CAROLINA FALASCHI; GREAVES, TAMAR LOUISE; BRANDAO PEREIRA, JORGE FERNANDO. Revealing a new fluorescence peak of the enhanced green fluorescent protein using three-dimensional fluorescence spectroscopy. RSC ADVANCES, v. 9, n. 40, p. 22853-22858, AUG 1 2019. (14/16424-7, 18/50009-8, 18/06576-5, 18/01858-2, 14/19793-3, 16/07529-5)

FERRARI, FELIPE AUGUSTO; BRANDAO PEREIRA, JORGE FERNANDO; WITKAMP, GEERT-JAN; SOARES FORTE, MARCUS BRUNO. Which Variables Matter for Process Design and Scale-Up? A Study of Sugar Cane Straw Pretreatment Using Low-Cost and Easily Synthesizable Ionic Liquids. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 15, p. 12779-12788, AUG 5 2019. (14/16424-7, 15/50612-8, 17/24520-4)

ALVES CAMELO, LIDIA CRISTINA; DIAS SANTOS, GABRIELA DE SOUZA; DE SOUZA, RANYERE LUCENA; FARIA SOARES, CLEIDE MARA; BRANDAO PEREIRA, JORGE FERNANDO; LIMA, ALVARO SILVA. Protic ionic liquids as constituent of aqueous two-phase system based on acetonitrile: Synthesis, phase diagrams and genipin pre-purification. Fluid Phase Equilibria, v. 507, MAR 1 2020. (14/16424-7)

CLAUDIO, ANA FILIPA M.; PEREIRA, JORGE F. B.; MCCRARY, PARKER D.; FREIRE, MARA G.; COUTINHO, JOAO A. P.; ROGERS, ROBIN D.. A critical assessment of the mechanisms governing the formation of aqueous biphasic systems composed of protic ionic liquids and polyethylene glycol. Physical Chemistry Chemical Physics, v. 18, n. 43, p. 30009-30019, NOV 21 2016. (14/16424-7)

PANAS, PAWEL; LOPES, CAMILA; CERRI, MARCEL O.; VENTURA, SONIA P. M.; SANTOS-EBINUMA, VALERIA C.; PEREIRA, JORGE F. B.. Purification of clavulanic acid produced by Streptomyces clavuligerus via submerged fermentation using polyethylene glycol/cholinium chloride aqueous two-phase systems. Fluid Phase Equilibria, v. 450, p. 42-50, OCT 25 2017. (14/16424-7, 14/01580-3)

DOS SANTOS, NATHALIA VIEIRA; SAPONI, CAROLINA FALASCHI; RYAN, TIMOTHY M.; PRIMO, FERNANDO L.; GREAVES, TAMAR L.; PEREIRA, JORGE F. B.. Reversible and irreversible fluorescence activity of the Enhanced Green Fluorescent Protein in pH: Insights for the development of pH-biosensors. International Journal of Biological Macromolecules, v. 164, p. 3474-3484, DEC 1 2020. (18/20833-0, 14/16424-7, 14/19793-3, 18/06576-5, 16/07529-5, 18/50009-8, 18/01858-2)

RODRIGUES, K. C. S.; COSTA, C. L. L.; BADINO, A. C.; PEDROLLI, D. B.; PEREIRA, J. F. B.; CERRI, M. O.. Application of Acid and Cold Stresses to Enhance the Production of Clavulanic Acid by Streptomyces clavuligerus. Applied Biochemistry and Biotechnology, v. 188, n. 3, p. 706-719, JUL 2019. (14/16424-7)

HIJO, ARIEL A. C. TOLEDO; MAXIMO, GUILHERME J.; CUNHA, ROSIANE L.; FONSECA, FELIPE H. S.; CARDOSO, LISANDRO P.; PEREIRA, JORGE F. B.; COSTA, MARIANA C.; BATISTA, EDUARDO A. C.; MEIRELLES, ANTONIO J. A.. Phase equilibrium and physical properties of biobased ionic liquid mixtures. Physical Chemistry Chemical Physics, v. 20, n. 9, p. 6469-6479, MAR 7 2018. (16/08566-1, 04/08517-3, 16/24461-5, 14/16424-7, 12/05027-1, 14/03992-7, 14/21252-0)

MAGRI, AGNES; PIMENTA, MARCELA V.; SANTOS, JOAO H. P. M.; COUTINHO, JOAO A. P.; VENTURA, SONIA P. M.; MONTEIRO, GISELE; RANGEL-YAGUI, CARLOTA O.; PEREIRA, JORGE F. B.. Controlling the l-asparaginase extraction and purification by the appropriate selection of polymer/salt-based aqueous biphasic systems. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 95, n. 4, p. 1016-1027, APR 2020. (13/08617-7, 18/15104-0, 14/16424-7, 18/25994-2, 14/19793-3, 15/07749-2)

PEREIRA, JORGE F. B.; FREIRE, MARA G.; COUTINHO, JOAO A. P.. Aqueous two-phase systems: Towards novel and more disruptive applications. Fluid Phase Equilibria, v. 505, FEB 1 2020. (14/16424-7)

MAGRI, AGNES; PECORARI, THAINA; PEREIRA, MATHEUS M.; CILLI, EDUARDO M.; GREAVES, TAMAR L.; PEREIRA, JORGE F. B.. Enhancing the Biocatalytic Activity of L-Asparaginase Using Aqueous Solutions of Cholinium-Based Ionic Liquids. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 24, p. 19720-19731, DEC 16 2019. (14/16424-7, 18/50009-8)

MUSSAGY, CASSAMO U.; PEREIRA, JORGE F. B.; DUFOSSE, LAURENT; RAGHAVAN, VIJAYA; SANTOS-EBINUMA, VALERIA C.; PESSOA JR, ADALBERTO. Advances and trends in biotechnological production of natural astaxanthin by Phaffia rhodozyma yeast. CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, AUG 2021. (17/50303-0, 15/50058-0, 14/19793-3, 14/16424-7, 18/06908-8, 20/08655-0)

NASCIMENTO, PALOMA A. M.; PICHELI, FLAVIO P.; LOPES, ANDRE M.; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C.. Effects of cholinium-based ionic liquids on Aspergillus niger lipase: Stabilizers or inhibitors. BIOTECHNOLOGY PROGRESS, v. 35, n. 5, SEP-OCT 2019. (14/16424-7, 14/01580-3)

FARIAS, FABIANE OLIVEIRA; PEREIRA, JORGE F. B.; COUTINHO, JOAO A. P.; IGARASHI-MAFRA, LUCIANA; MAFRA, MARCOS R.. Understanding the role of the hydrogen bond donor of the deep eutectic solvents in the formation of the aqueous biphasic systems. Fluid Phase Equilibria, v. 503, JAN 1 2020. (14/16424-7, 18/50009-8)

BARROS, HELENA D. F. Q.; MAXIMO, GUILHERME J.; CAZARIN, CINTHIA B. B.; PEREIRA, JORGE F. B.; MAROSTICA JUNIOR, MARIO R.. Subacute toxicity assessment of biobased ionic liquids in rats. Food Research International, v. 134, AUG 2020. (14/21252-0, 16/08566-1, 16/24461-5, 17/04231-8, 14/16424-7)

LOPES, CAMILA; DOS SANTOS, NATHALIA VIEIRA; DUPONT, JANA; PEDROLLI, DANIELLE BISCARO; VALENTINI, SANDRO ROBERTO; SANTOS-EBINUMA, VALERIA DE CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Improving the cost effectiveness of enhanced green fluorescent protein production using recombinant Escherichia coli BL21 (DE3): Decreasing the expression inducer concentration. Biotechnology and Applied Biochemistry, v. 66, n. 4, p. 527-536, JUL 2019. (14/16424-7, 18/50009-8, 14/19793-3, 16/07529-5)

MUSSAGY, CASSAMO U.; SANTOS-EBINUMA, VALERIA C.; GONZALEZ-MIQUEL, MARIA; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.. Protic Ionic Liquids as Cell-Disrupting Agents for the Recovery of Intracellular Carotenoids from Yeast Rhodotorula glutinis CCT-2186. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 19, p. 16765-16776, OCT 7 2019. (14/16424-7, 14/01580-3, 14/19793-3, 17/50303-0)

MUSSAGY, CASSAMO USSEMANE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Production and extraction of carotenoids produced by microorganisms. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1095-1114, FEB 2019. (17/50303-0, 14/16424-7, 14/01580-3, 14/19793-3)

TORRES-ACOSTA, MARIO A.; PEREIRA, JORGE F. B.; FREIRE, MARA G.; AGUILAR-YANEZ, JOSE M.; COUTINHO, JOAO A. P.; TITCHENER-HOOKER, NIGEL J.; RITO-PALOMARES, MARCO. Economic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systems. Separation and Purification Technology, v. 203, p. 178-184, SEP 12 2018. (14/16424-7)

SILVA, FRANCISCA A. E; PEREIRA, JORGE F. B.; KURNIA, KIKI A.; VENTURA, SONIA P. M.; SILVA, ARTUR M. S.; ROGERS, ROBIN D.; COUTINHO, JOAO A. P.; FREIRE, MARA G.. Temperature dependency of aqueous biphasic systems: an alternative approach for exploring the differences between Coulombic-dominated salts and ionic liquids. CHEMICAL COMMUNICATIONS, v. 53, n. 53, p. 7298-7301, JUL 7 2017. (14/16424-7)

V. VERISSIMO, NATHALIA; SAPONI, CAROLINA F.; RYAN, TIMOTHY M.; GREAVES, TAMAR L.; PEREIRA, JORGE F. B.. Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity. GREEN CHEMICAL ENGINEERING, v. 2, n. 4, p. 11-pg., 2021-12-01. (18/06576-5, 18/25511-1, 14/16424-7, 20/14144-8, 18/50009-8, 14/19793-3, 18/01858-2, 18/20833-0, 16/07529-5)

MUSSAGY, CASSAMO USSEMANE; CARIZIA GUIMARAES, ANA ALICE; FERREIRA ROCHA, LARA VICENTE; WINTERBURN, JAMES; SANTOS-EBINUMA, VALERIA DE CARVALHO; BRANDAO PEREIRA, JORGE FERNANDO. Improvement of carotenoids production from Rhodotorula glutinis CCT-2186. Biochemical Engineering Journal, v. 165, JAN 15 2021. (17/50303-0, 14/16424-7, 14/01580-3, 14/19793-3, 19/09618-3)

VERISSISMO, NATHALIA V.; NAKAMURA, CECILIA NAOMI; DE OLIVEIRA, FERNANDA; KUHN, BRUNA L.; FRIZZO, CLARISSA P.; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C.. Effect of amphiphilic ionic liquids on the colorimetric properties of polyketides colorants. JOURNAL OF MOLECULAR LIQUIDS, v. 363, p. 11-pg., 2022-07-30. (14/01580-3, 14/16424-7, 21/09175-4, 19/15493-9, 21/06686-8)

MARTINS, MARGARIDA; OOI, CHIEN WEI; NEVES, MARCIA C.; PEREIRA, JORGE F. B.; COUTINHO, JOAO A. P.; VENTURA, SONIA P. M.. Extraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compounds. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, p. 7-pg., 2018-07-01. (14/16424-7)

PEREIRA, JORGE F. B.; KURNIA, KIKI A.; FREIRE, MARA G.; COUTINHO, JOAO A. P.; ROGERS, ROBIN D.. Controlling the Formation of Ionic-Liquid-based Aqueous Biphasic Systems by Changing the Hydrogen-Bonding Ability of Polyethylene Glycol End Groups. ChemPhysChem, v. 16, n. 10, p. 2219-2225, JUL 20 2015. (14/16424-7)

LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; DOS SANTOS-EBINUMA, VALERIA CARVALHO; PESSOA, JR., ADALBERTO; VALENTINI, SANDRO ROBERTO; BRANDAO PEREIRA, JORGE FERNANDO. Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 1. Polymer-polymer systems. Separation and Purification Technology, v. 206, p. 39-49, NOV 29 2018. (05/60159-7, 14/16424-7, 14/19793-3, 07/51978-0)

MARTINS NASCIMENTO, PALOMA ANDRADE; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Insights into the effect of imidazolium-based ionic liquids on chemical structure and hydrolytic activity of microbial lipase. Bioprocess and Biosystems Engineering, v. 42, n. 7, p. 1235-1246, JUL 2019. (14/16424-7)

PEREIRA, JORGE F. B.; BARBER, PATRICK S.; KELLEY, STEVEN P.; BERTON, PAULA; ROGERS, ROBIN D.. Double salt ionic liquids based on 1-ethyl-3-methylimidazolium acetate and hydroxyl-functionalized ammonium acetates: strong effects of weak interactions. Physical Chemistry Chemical Physics, v. 19, n. 39, p. 26934-26943, OCT 21 2017. (14/16424-7)

NEVES, CATARINA M. S. S.; SHAHRIARI, SHAHLA; LEMUS, JESUS; PEREIRA, JORGE F. B.; FREIRE, MARA G.; COUTINHO, JOAO A. P.. Aqueous biphasic systems composed of ionic liquids and polypropylene glycol: insights into their liquid-liquid demixing mechanisms. Physical Chemistry Chemical Physics, v. 18, n. 30, p. 20571-20582, AUG 14 2016. (14/19793-3, 14/16424-7)

DOS SANTOS, NATHALIA VIEIRA; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; BRANDAO PEREIRA, JORGE FERNANDO. Liquid-liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 7, p. 19-pg., 2018-07-01. (14/16424-7, 14/01580-3, 16/07529-5, 13/08617-7, 14/19793-3)

NAKAMURA, CECILIA NAOMI; PORPHIRIO VERISSIMO, NATHALIA VIEIRA; OLIVEIRA, FERNANDA; FRIZZO, CLARISSA P.; PEREZ-SANCHEZ, GERMAN; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA G.. Extraction of natural colorants using supramolecular solvents composed of Triton X-114 and ionic liquids. Separation and Purification Technology, v. 319, p. 10-pg., 2023-05-20. (21/06686-8, 19/15493-9, 21/09175-4, 14/16424-7)

MUSSAGY, CASSAMO U.; TABANEZ, NAIARA L.; FARIAS, FABIANE O.; KURNIA, KIKI A.; MAFRA, MARCOS R.; PEREIRA, JORGE F. B.. Determination, characterization and modeling of aqueous biphasic systems composed of propylammonium-based ionic liquids and phosphate salts. Chemical Physics Letters, v. 754, SEP 2020. (15/50058-0, 14/16424-7, 14/19793-3)

MUSSAGY, CASSAMO U.; SANTOS-EBINUMA, VALERIA C.; KURNIA, KIKI A.; DIAS, V, ANA C. R.; CARVALHO, PEDRO; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.. Integrative platform for the selective recovery of intracellular carotenoids and lipids from Rhodotorula glutinis CCT-2186 yeast using mixtures of bio-based solvents. GREEN CHEMISTRY, v. 22, n. 23, p. 8478-8494, DEC 7 2020. (14/16424-7, 15/50058-0, 14/19793-3, 17/50303-0)

DE OLIVEIRA, FERNANDA; HIRAI, PATRICIA RIE; SIMAS TEIXEIRA, MARIA FRANCISCA; PEREIRA, JORGE F. B.; SANTOS-EBINUMA, VALERIA C.. Talaromyces amestolkiae cell disruption and colorant extraction using imidazolium-based ionic liquids. Separation and Purification Technology, v. 257, FEB 15 2021. (14/16424-7, 14/01580-3)

SOARES, BRUNA P.; SANTOS, JOAO H. P. M.; MARTINS, MARGARIDA; ALMEIDA, MAFALDA R.; SANTOS, V, NATHALIA; FREIRE, MARA G.; SANTOS-EBINUMA, VALERIA C.; COUTINHO, JOAO A. P.; PEREIRA, JORGE F. B.; VENTURA, SONIA P. M.. Purification of green fluorescent protein using fast centrifugal partition chromatography. Separation and Purification Technology, v. 257, FEB 15 2021. (14/16424-7, 18/25994-2, 16/07529-5)

KURNIK, ISABELLE S.; MUSSAGY, CASSAMO U.; PEREIRA, JORGE F. B.; LOPES, ANDRE M.. Amphiphilic copolymer aqueous solutions with cholinium ionic liquids as adjuvants: New insights into determination of binodal curves and phase-separation mechanisms. JOURNAL OF MOLECULAR LIQUIDS, v. 318, NOV 15 2020. (14/16424-7, 18/05111-9, 19/05624-9, 17/10789-1, 18/10799-0, 19/08549-8)

DOS SANTOS, NATHALIA V.; MARTINS, MARGARIDA; SANTOS-EBINUMA, VALERIA C.; VENTURA, SONIA P. M.; COUTINHO, JOAO A. P.; VALENTINI, SANDRO R.; PEREIRA, JORGE F. B.. Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 6, n. 7, p. 9383-9393, JUL 2018. (14/16424-7, 14/19793-3, 16/07529-5)

TOLEDO HIJO, ARIEL A. C.; MAXIMO, GUILHERME J.; COSTA, MARIANA C.; CUNHA, ROSIANE L.; PEREIRA, JORGE F. B.; KURNIA, KIKI A.; BATISTA, EDUARDO A. C.; MEIRELLES, ANTONIO J. A.. Phase Behavior and Physical Properties of New Biobased Ionic Liquid Crystals. Journal of Physical Chemistry B, v. 121, n. 14, p. 3177-3189, APR 13 2017. (14/16424-7, 07/58017-5, 14/21252-0, 12/05027-1, 16/08566-1, 14/03992-7)

KURNIK, ISABELLE S.; NORONHA, MARIANA A.; CAMARA, MAYRA C. C.; MAZZOLA, PRISCILA G.; VICENTE, ANTONIO A.; PEREIRA, JORGE F. B.; LOPES, ANDRE M.. Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids. Separation and Purification Technology, v. 250, NOV 1 2020. (14/16424-7, 17/10789-1, 19/05624-9, 18/05111-9, 19/08549-8, 18/10799-0)

KURNIK, ISABELLE S.; D'ANGELO, NATALIA A.; MAZZOLA, PRISCILA G.; CHORILLI, MARLUS; KAMEI, DANIEL T.; PEREIRA, JORGE F. B.; VICENTE, ANTONIO A.; LOPES, ANDRE M.. Polymeric micelles using cholinium-based ionic liquids for the encapsulation and release of hydrophobic drug molecules. BIOMATERIALS SCIENCE, v. 9, n. 6, p. 2183-2196, MAR 21 2021. (18/10799-0, 20/03727-2, 14/16424-7, 18/05111-9, 19/05624-9, 17/10789-1, 19/08549-8)

PEREZ-SANCHEZ, GERMAN; SCHAEFFER, NICOLAS; LOPES, ANDRE M.; PEREIRA, JORGE F. B.; COUTINHO, JOAO A. P.. Using coarse-grained molecular dynamics to understand the effect of ionic liquids on the aggregation of Pluronic copolymer solutions. Physical Chemistry Chemical Physics, v. 23, n. 10, p. 5824-5833, MAR 14 2021. (18/10799-0, 14/16424-7, 17/10789-1)