Genotypic diversity and presence of beta-lactamase encoding genes in Pseudomonas aeruginosa isolated from Brazilian soils

Autor(es)

Braz, Vania Santos ^[1] ; Rueda Furlan, Joao Pedro ^[1] ; Passaglia, Jaqueline ^[1] ; Falcao, Juliana Pfrimer ^[1] ; Stehling, Eliana Guedes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: APPLIED SOIL ECOLOGY; v. 129, p. 94-97, AUG 2018.
• Citações Web of Science: 1

Resumo

Pseudomonas aeruginosa is considered an opportunistic pathogen widely distributed in the environment, which has a high genetic diversity among the isolates due to the frequent genetic recombination. This study determined the genetic relatedness among 61 Pseudomonas aeruginosa isolated from soil of different Brazilian regions by pulsed-field gel electrophoresis (PFGE), investigated the resistance profile and the prevalence of beta-lactamase encoding genes in these isolates. It was observed a high genotypic diversity among the majority of the isolates (>= 33.6%), which were differentiated in 55 PFGE-types. However, some few isolates presented a high similarity (>= 80%) among each other, being allocated in B1-B8 subgroups. The majority of the isolates showed non-susceptibility to aztreonam (16 to >= 256 mu gmL(-1)) and 38% of the isolates had one or more of the target genes, being bla(SHV), bla(GES) and bla(VEB) the most prevalent. This work provided new information about the genotypic diversity and antimicrobial resistance mechanisms of P. aeruginosa isolated from soils of different states in Brazil. (AU)

• Processo FAPESP: 15/18990-2 - Investigação dos mecanismos de resistência às quinolonas em isolados bacterianos ambientais
• Beneficiário: Eliana Guedes Stehling
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/24716-3 - Sequenciamento do genoma, transcriptoma e análise fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular