Development of a reusable and sustainable biocatalyst by immobilization of soybean peroxidase onto magnetic adsorbent

Autor(es)

Torres, J. A. ^[1] ; Silva, M. C. ^[2] ; Lopes, J. H. ^[3] ; Nogueira, A. E. ^[4] ; Nogueira, F. G. E. ^[5] ; Correa, A. D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Lavras, Dept Chem, BR-37200000 Lavras - Brazil

^[2] Univ Fed Sao Joao Del Rei, Dept Chem, BR-31270901 Sao Joao Del Rei - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Lab Engn & Prod Chem LEQUIP, Dept Mat Engn & Bioproc DEMBIO, Sch Chem Engn FEQ, BR-13083852 Campinas, SP - Brazil

^[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil

^[5] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 114, p. 1279-1287, JUL 15 2018.
• Citações Web of Science: 4

Resumo

In this work we synthesized an activated carbon/magnetite composite by a simple co-precipitation method. The activated carbon (AC) was synthesized from the solid waste obtained in the extraction process of the peroxidase enzyme and the magnetic composite was used as support for the immobilization of soybean peroxidase (SP). After the determination of the optimal immobilization parameters, a 100% yield was achieved under the following conditions: support:enzyme proportion of 1.0:0.05 g, equilibration time of 7 h, pH 3.0 (citrate buffer phosphate 0.1 mol L-1) and temperature of 50 degrees C. The determination of pH to the point of zero charge was also done to assist in the understanding of the immobilization process at different pH values. Several characterization techniques were used, such as thermogravimetric analysis, elemental analysis composition, X-ray powder diffraction, Fourier transform infrared spectroscopy and Scanning electron microscopy. The biocatalyst presented excellent operational stability and was reused for 11 consecutive cycles. The magnetic properties inserted in the AC contributed to the removal of the biocatalyst from the reaction medium without interfering in the adsorptive characteristics of the AC. Thus, the activated carbon/magnetite composite can be applied to different research fields with high performance. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/09014-7 - Estudo do potencial de nano-heteroestruturas de óxidos de cobre e nióbio para aplicação em fotossíntese artificial
• Beneficiário: André Esteves Nogueira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado