Challenges for the Self-Assembly of Poly(Ethylene Glycol)-Poly(Lactic Acid) (PEG-PLA) into Polymersomes: Beyond the Theoretical Paradigms

Autor(es)

Apolinario, Alexsandra Conceicao ^[1] ; Magon, Monika S. ^{[2, 3]} ; Pessoa Jr, Adalberto ; Rangel-Yagui, Carlota de Oliveira ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Prof Lineu Prestes 580-B1-16, BR-05508000 Sao Paulo - Brazil

^[2] UCL, Dept Chem, Christopher Ingold Bldg, 20 Gordon St, London WC1H 0AJ - England

^[3] Univ St Andrews, Sch Biol, BSRC Complex, St Andrews KY16 9ST, Fife - Scotland

^[4] Pessoa Jr, Jr., Adalberto, Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Prof Lineu Prestes 580-B1-16, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NANOMATERIALS; v. 8, n. 6 JUN 2018.
• Citações Web of Science: 3

Resumo

Polymersomes (PL), vesicles formed by self-assembly of amphiphilic block copolymers, have been described as promising nanosystems for drug delivery, especially of biomolecules. The film hydration method (FH) is widely used for PL preparation, however, it often requires long hydration times and commonly results in broad size distribution. In this work, we describe the challenges of the self-assembly of poly (ethylene glycol)-poly(lactic acid) (PEG-PLA) into PL by FH exploring different hydrophilic volume fraction (f) values of this copolymer, stirring times, temperatures and post-FH steps in an attempt to reduce broad size distribution of the nanostructures. We demonstrate that, alongside f value, the methods employed for hydration and post-film steps influence the PEG-PLA self-assembly into PL. With initial FH, we found high PDI values (>0.4). However, post-hydration centrifugation significantly reduced PDI to 0.280. Moreover, extrusion at higher concentrations resulted in further improvement of the monodispersity of the samples and narrow size distribution. For PL prepared at concentration of 0.1% (m/v), extrusion resulted in the narrower size distributions corresponding to PDI values of 0.345, 0.144 and 0.081 for PEG(45)-PLA(69), PEG(114)-PLA(153) and PEG(114)-PLA(180), respectively. Additionally, we demonstrated that copolymers with smaller f resulted in larger PL and, therefore, higher encapsulation efficiency (EE%) for proteins, since larger vesicles enclose larger aqueous volumes. (AU)

• Processo FAPESP: 13/08617-7 - Produção de L-asparaginase extracelular: da bioprospecção à engenharia de um biofármaco antileucêmico
• Beneficiário: Adalberto Pessoa Junior
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/10456-4 - Desenvolvimento e caracterização de Polimerossomos de poli (óxido de Etileno-beta-ácido láctico) (PEG-PLA) para veiculação de L-asparaginase recombinante
• Beneficiário: Alexsandra Conceição Apolinário
• Linha de fomento: Bolsas no Brasil - Doutorado