Timing carbon turnover (δ13C) in weaned piglet’s brain by IRMS

• Tipo de documento: Artigo Científico
• Fonte: Anais da Academia Brasileira de Ciências; n. ahead, p. -, 2018.

Resumo

Abstract Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (δ13C) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and adjusted to the first-order equation by a nonlinear regression analysis using NLIN procedure of SAS, in order to obtain the exponential graphics. The carbon turnover (t95%) verified for cerebral tissue was faster (P<0.05) for diet containing glutamate in comparison to other diets, supporting the fact that glutamate contributed to develop the piglets’ brain, due to the fastest incorporation of 13C-atoms in this tissue at post-weaning phase, despite the energy deficit experienced by them. (AU)

• Processo FAPESP: 13/25616-4 - Capacidade de adaptação de leitões ao desmame recebendo dietas suplementadas com nucleotídeos e ácido glutâmico
• Beneficiário: Dirlei Antonio Berto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/13169-6 - Troca isotópica do carbono (´13C) em órgãos e músculos de leitões recebendo dietas com nucleotídeos e glutamato
• Beneficiário: Mayra Anton Dib Saleh
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado