Citogenotoxic response of juvenile cobia Rachycentron canadum (Linnaeus, 1766) reared in two different systems

Autor(es)

Botelho, Marina Tenorio ^[1] ; Campos Rocha Passos, Maria Jose de Arruda ^[1] ; Hasue, Fabio Matsu ^[1] ; Lemos, Daniel ^[1] ; Rodrigues, Edson ^[2] ; Yasumaru, Fanny ^[1] ; Phan, Ngan Van ; Gomes, Vicente ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Oceanog, Praca Oceanog 191, BR-05508120 Butanta, SP - Brazil

^[2] Univ Taubate, Dept Biol, Av Tiradentes, 500 Jardim das Nacoes, BR-12030180 Taubate, SP - Brazil

^[3] Ngan Van Phan, Univ Sao Paulo, Inst Oceanog, Praca Oceanog 191, BR-05508120 Butanta, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Oceanography; v. 66, n. 2, p. 189-198, APR-JUN 2018.
• Citações Web of Science: 0

Resumo

Abstract Aquaculture production is continuously growing worldwide, and marine fish farming in Brazil is still in its infancy. Intensive farming conditions may cause physiological stress to the cultured organism, which can be evaluated by citogenotoxic biomarkers. The aim of this study was to assess the genotoxic effect of the rearing conditions in red blood cells of juvenile cobia Rachycentron canadum by using comet assay and micronucleus and other nuclear abnormalities assay. Juvenile cobia were reared for 13 weeks in indoor tank with open water circulation and in near shore cage. The comet assay and the nuclear abnormalities assay detected higher DNA damage and higher nuclear abnormalities frequency in erythrocytes of fish reared in the indoor tank. Results showed that two methods are complementary. Additionally, cobia were injected with ß-naphthoflavone (BNF) at concentrations of 2mgkg-1 and 10mgkg-1 in laboratory controlled conditions, and maintained for 7 days in separate tanks to better understand the response mechanisms of this species to a toxic substance. The comet assay did not detect any significant differences between BNF injected and control fish, whereas nuclear abnormalities assay showed significant differences between BNF injected and the control groups. The damages identified by the comet assay are repairable breaks in the DNA strands, whereas nuclear abnormalities may be permanent. Possibly the period of maintenance after injection was enough to clean BNF from the organisms and to repair the breaks in the DNA strands. As cobia seems to respond very well to genotoxic elements, comet assay and nuclear abnormalities assay would be useful tools to monitor farming conditions. (AU)

• Processo FAPESP: 16/10772-9 - Criação do bijupirá (Rachycentron canadum) no litoral Sudeste do Brasil (SP, RJ) durante a fase de engorda: alimentação e nutrição, interações com o ambiente e a economia em modelos existentes (PeixeMar)
• Beneficiário: Daniel Eduardo Lavanholi de Lemos
• Modalidade de apoio: Auxílio à Pesquisa - Regular