Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp.

Autor(es)

Garcia, Leandro Encarnacao ^[1] ; de Araujo Junior, Erivelto Correa ^[1] ; Melo, Larissa Martins ^[2] ; Bragato, Jaqueline Poleto ^[2] ; Peiro, Juliana Regina ^[2] ; Felix de Lima, Valeria Marcal ^[2] ; Marinho, Marcia ^[2] ; Arnold, Daniel Robert ^[1] ; Lopes, Flavia Lombardi ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Support Prod & Anim Hlth, Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 12, n. 7 JUL 2018.
• Citações Web of Science: 0

Resumo

Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A. 1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis. (AU)

• Processo FAPESP: 14/20719-2 - Perfil da atividade de macrófagos in vitro, frente à amostra virulenta, atenuada e saprófita de Leptospira spp.
• Beneficiário: Márcia Marinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/14229-5 - Caracterização do microtranscriptoma de macrófagos em resposta à exposição a cepas virulenta, atenuada e saprófita de Leptospira spp
• Beneficiário: Flavia Lombardi Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Regular