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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp.

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Author(s):
Garcia, Leandro Encarnacao [1] ; de Araujo Junior, Erivelto Correa [1] ; Melo, Larissa Martins [2] ; Bragato, Jaqueline Poleto [2] ; Peiro, Juliana Regina [2] ; Felix de Lima, Valeria Marcal [2] ; Marinho, Marcia [2] ; Arnold, Daniel Robert [1] ; Lopes, Flavia Lombardi [1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Support Prod & Anim Hlth, Aracatuba, SP - Brazil
[2] Sao Paulo State Univ Unesp, Sch Vet Med, Dept Clin Surg & Anim Reprod, Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 12, n. 7 JUL 2018.
Web of Science Citations: 0
Abstract

Leptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A. 1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis. (AU)

FAPESP's process: 14/20719-2 - Profile of macrophage activity in vitro against the virulent, attenuated and saprophytic Leptospira spp. sample
Grantee:Márcia Marinho
Support Opportunities: Regular Research Grants
FAPESP's process: 15/14229-5 - Characterization of the microtranscriptoma of macrophages in response to virulent, attenuated and saprophytic strains of Leptospira spp
Grantee:Flavia Lombardi Lopes
Support Opportunities: Regular Research Grants