Profile of macrophage activity in vitro against the virulent, attenuated and saprophytic Leptospira spp. sample

• Grant number: 14/20719-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Márcia Marinho
• Grantee: Márcia Marinho
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The genus Leptospira brings pathogenic species, L. interrogans and saprophytic, L. biflexa and intermediates with varying degrees of pathogenicity. It is known that there is a mobile cellular immune response with activation of macrophages expressing genes responsible for the production of cytokines involved in the pathogenesis of leptospirosis during the infectious process. The cytotoxicity has been reported in previous studies that consider the L. interrogans as an inducing agent of apoptosis of macrophages and hepatocytes. However, the mechanism responsible for cell death remains unclear. This study aims to determine the dynamics of the cellular immune response in vitro using cultures of macrophages (J774) when exposed to virulent strains, attenuated saprophytic Leptospira, observed at various times after exposure. To analyze the activation of macrophage cultures in vitro assays to determine the production of reactive nitrogen intermediates (NO), expression of genes for cytokines and interleukins by PCR and the presence and quantify them in the supernatant of cell culture will be performed by ELISA. For these reasons, we seek to verify and compare the kinetics of response of macrophage activity when exposed to Leptospira spp to the pathogenic, attenuated and saprófito inoculum in order to understand the dynamics of immune cell motility. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, LEANDRO ENCARNACAO; DE ARAUJO JUNIOR, ERIVELTO CORREA; MELO, LARISSA MARTINS; BRAGATO, JAQUELINE POLETO; PEIRO, JULIANA REGINA; FELIX DE LIMA, VALERIA MARCAL; MARINHO, MARCIA; ARNOLD, DANIEL ROBERT; LOPES, FLAVIA LOMBARDI. Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp.. PLoS Neglected Tropical Diseases, v. 12, n. 7, JUL 2018. (14/20719-2, 15/14229-5)

DE ARAUJO JUNIOR, ERIVELTO CORREA; GARCIA, LEANDRO ENCARNACAO; MELO, LARISSA MARTINS; BRAGATO, JAQUELINE POLETO; FELIX DE LIMA, VALERIA MARCAL; PEIRO, JULIANA REGINA; LOPES, FLAVIA LOMBARDI; MARINHO, MARCIA. Transcriptome datasets of macrophages infected with different strains of Leptospira spp. DATA IN BRIEF, v. 16, p. 1044-1050, FEB 2018. (14/20719-2, 15/14229-5)

DE ARAUJO JUNIOR, ERIVELTO CORREA; GARCIA, LEANDRO ENCARNACAO; ARAUJO, MATHEUS JANECK; OLIVEIRA-JUNIOR, ITAMAR SOUZA; ARNOLD, DANIEL ROBERT; LOPES, FLAVIA LOMBARDI; MARINHO, MARCIA. Gene expression is associated with virulence in murine macrophages infected with Leptospira spp. PLoS One, v. 14, n. 12, DEC 4 2019. (14/20719-2)