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Profile of macrophages in vitro activity, compared to the sample virulent, attenuated and saprophytic Leptospira spp

Grant number: 15/11855-2
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Márcia Marinho
Grantee:Reinaldo Augusto Ferreira Victor
Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The genus Leptospira brings pathogenic species, L. interrogans and saprophytic, L. biflexa with varying degrees and intermediate pathogenicity. It is known that during leptospirosis there is a mobile cellular immune response with activation of macrophages expressing genes responsible for the production of cytokines that participate in the pathogenesis of the infection. The cytotoxicity has been reported in previous studies that consider the L. interrogans as an inducer of apoptosis in macrophages and hepatocytes. However, the mechanism responsible for cell death remains unclear. This study aims to determine the dynamics of the cellular immune response in vitro using macrophage cultures (J774) when exposed to virulent strains, attenuated and saprophytic Leptospira, observed at various times after exposure. To analyze the activation of macrophages in vitro cultivation will be carried out tests which determine the production of reactive nitrogen intermediates (NO) and quantification of cytokines in the cell culture supernatant using the ELISA test. For these reasons, it seeks to check and compare the response kinetics of macrophage activity when exposed to Leptospira spp, against pathogenic inoculum, attenuated and saprófito in order to understand the dynamics of cellular immune mobility.

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