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Searching for genes potentially involved in virulence control in Leishmania major

Grant number: 13/10294-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2013
Effective date (End): December 31, 2014
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Angela Kaysel Cruz
Grantee:Paula Andrea Castaneda Londono
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Human infections with Leishmania spp give rise to a spectrum of clinical manifestations ranging from tegumentary lesions to a visceral disease. In this context, the identification / characterization of genes or groups of genes that affect the pattern of virulence of the parasite is important to improve understanding of the pathogenic mechanisms. Previous studies conducted in our laboratory described the attenuation of virulence due to overexpression of the SL RNA in transfectants of Leishmania (L.) major and Leishmania (V.) braziliensis in a murine model. Evaluation of global changes in gene expression profile was conducted to understand the phenotype. These comparative analyses allowed the identification of genes that were over- or under-expressed on the attenuated mutants; these genes may individually contribute to the virulence behavior. To verify this hypothesis we will investigate the effect of a group of these genes (three to six) found as under- or over-expressed. The genes will be cloned into a Leishmania expression vector and transfected into two L. (L.) major strains (virulent and nonvirulent strains in susceptible mice) to analyze the infection pattern in vitro and in vivo using peritoneal macrophages and susceptible mice, BALB/c.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LONDONO, Paula Andrea Castaneda. Searching for genes potentially involved in virulence control in Leishmania major.. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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