| Texto completo | |
| Autor(es): |
Carvalho, Pedro L. P. F.
[1, 2, 3]
;
Yamamoto, Fernando Y.
[2]
;
Barros, Margarida M.
[1]
;
Gatlin, III, Delbert M.
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista, Dept Melhoramento & Nutr Anim, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
[2] III, Texas A&M Univ Syst, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 - USA
[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Zootecnia, Ave Padua Dias, 11, CP 9, BR-13418900 Piracicaba, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FISH & SHELLFISH IMMUNOLOGY; v. 80, p. 592-599, SEP 2018. |
| Citações Web of Science: | 1 |
| Resumo | |
Under appropriate conditions, glutamine (Gln) is an essential nutrient for immunological responses, acting as a metabolic substrate for proliferation of enterocytes and lymphocytes, and having positive effects on the function of stimulated immune cells. Thus, specific components of both innate and adaptive immune systems of Nile tilapia were evaluated after supplementing Gln to cell culture media. Primary cell cultures of kidney leukocytes were used for respiratory burst and phagocytic activity assessment. The ability of macrophages to kill Streptococcus iniae also was evaluated. Additionally, a proliferation assay was conducted with peripheral blood lymphocytes (PBL) exposed to non-specific mitogens. Results showed that macrophage phagocytosis, anion superoxide production, and bactericidal capacity were significantly (P < 0.05) enhanced by Gin supplementation to the culture media. The proliferation of lymphocytes upon mitogenic exposure also was significantly (P < 0.05) enhanced by Gin supplementation to the media. Our results suggest that in vitro, different levels of Gin were necessary for optimal immunological responses of leukocytes and lymphocytes. As such, Gin supplementation was able to enhance and modulate both innate and adaptive responses of Nile tilapia leukocytes, highlighting its potential application as an immunonutrient. (AU) | |
| Processo FAPESP: | 15/23777-6 - Suplementação dietética de glutamina e desempenho produtivo, morfologia intestinal, respostas hemáticas, enzimáticas e imunológicas da tilápia-do-Nilo submetida a desafio bacteriano |
| Beneficiário: | Pedro Luiz Pucci Figueiredo de Carvalho |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 14/01973-5 - Suplementação dietética de glutamina e desempenho produtivo, morfologia intestinal, respostas hemáticas, enzimáticas e imunológicas da tilápia-do-Nilo submetida a desafio bacteriano |
| Beneficiário: | Pedro Luiz Pucci Figueiredo de Carvalho |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |