Characterization of monocyte-derived dendritic cells used in immunotherapy for HIV-1-infected individuals

Autor(es)

da Silva, Lais Teodoro ^[1] ; da Silva, Wanessa Cardoso ^[1] ; de Almeida, Alexandre ^[1] ; Reis, Denise da Silva ^[1] ; Santllo, Bruna Tereso ^[1] ; Rigato, Paula Ordonhez ^[2] ; da Silva Duarte, Alberto Jose ^[1] ; Oshiro, Telma Miyuki ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Dermatol & Imunodeficiencias, BR-05403903 Sao Paulo, SP - Brazil

^[2] Adolfo Lutz Inst, BR-01246000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Immunotherapy; v. 10, n. 10, p. 871-885, AUG 2018.
• Citações Web of Science: 2

Resumo

Aims: A therapeutic vaccine based on monocyte-derived dendritic cells (MDDCs) has been shown to represent a promising strategy for the treatment of cancer and viral infections. Here, we characterized the MDDCs used as an immunogen in a clinical trial for an anti-HIV-1 therapeutic vaccine. Patients \& Methods: Monocytes obtained from 17 HIV-infected individuals were differentiated into MDDCs and, after loading with autologous HIV, the cells were characterized concerning surface molecule expression, migratory and phagocytosis capacity, cytokine production and the induction of an effective cell-mediated immune response. Results: The MDDCs were able to induce antigen-specific responses in autologous CD4+ and CD8+ T lymphocytes. Conclusions: Despite a large interindividual variability, the results suggested that MDDCs present the potential to promote immune responses in vaccinated patients. (AU)

• Processo FAPESP: 12/18879-6 - Caracterização das células dendríticas utilizadas em um ensaio clínico de Fase I/II de vacina terapêutica anti-HIV
• Beneficiário: Telma Miyuki Oshiro Sumida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17822-0 - Caracterização das células dendríticas utilizadas em um ensaio clínico de fase I/II de vacina terapêutica anti-HIV
• Beneficiário: Laís Teodoro da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto