Characterization of PNIPAAm-co-AAm hydrogels for modified release of bromelain

Autor(es):Mostrar menos -

Croisfelt, Fernanda Machado ^[1] ; Ataide, Janaina Artem ^[2] ; Tundisi, Louise Lacalendola ^[3] ; Cefali, Leticia Caramori ^[1] ; Rebelo, Marcia de Araujo ^[4] ; Davila Sanchez, Jose Luis ^[5] ; da Costa, Tais Germano ^[6] ; Lima, Renata ^[6] ; Jozala, Angela Faustino ^[7] ; Chaud, Marco Vinicius ^[4] ; d'Avila, Marcos Akira ^[5] ; Tambourgi, Elias Basile ^[8] ; Silveira, Edgar ^[9] ; Mazzola, Priscila Gava ^[10]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Grad Program Biosci & Technol Bioact Prod, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Med Sci, Grad Program Med Sci, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, Grad Program Pharmaceut Sci, Campinas, SP - Brazil

^[4] Univ Sorocaba UNISO, Lab Biomat & Nanotechnol, Sorocaba, SP - Brazil

^[5] Univ Estadual Campinas, Sch Mech Engn, Campinas, SP - Brazil

^[6] Univ Sorocaba UNISO, Bioact Assessment & Toxicol Nanomat LABiToN, Sorocaba, SP - Brazil

^[7] Univ Sorocaba UNISO, Lab Ind Microbiol & Fermentat Proc LaMInFe, Sorocaba, SP - Brazil

^[8] Univ Campinas UNICAMP, Sch Chem Engn, Campinas, SP - Brazil

^[9] Fed Univ Uberlandia UFU, Inst Biotechnol, Uberlandia, MG - Brazil

^[10] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN POLYMER JOURNAL; v. 105, p. 48-54, AUG 2018.
• Citações Web of Science: 4

Resumo

Thermosensitive materials, such as PNIPAAm, are considered for the development of hydrogels for controlled release, especially for topical use. Bromelain is a set of proteases found in plants (Bromeliceae family) and it is known for having several medical uses, including healing of topical wounds. The combination of PNIPAAm hydrogels and bromelain could result in a potential formulation for topical injuries. In this work, different NIPAAm hydrogels were submitted to swelling tests for 24 h. The selected formulation presented a swelling ratio of 340%, and was used for bromelain loading, reaching up to 30% of protein loading. After loading, hydrogel disks were dried and immersed in phosphate buffer (pH 7.0) for release study. Release studies pointed to an initial burst release, followed by a modified (slower) release, reaching its optimum enzymatic activity release rates at around 60 min in both temperatures (25 degrees C and 37 degrees C). Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed bromelain loading through hydrogen bonds, without interfering in intermolecular polymeric chains. Bromelain presence decreased mucoadhesion at 25 degrees C and increased at 37 degrees C; it also increases polymers crosslink, without interfering in elastic properties (drilling and resilience). Hydrogels (blank and loaded with bromelain) showed a typical behavior of crosslinked hydrogels at rheology tests, and bromelain increased storage modulus. Both hydrogels showed a significant cell death after 24 h in NIH/3T3 cell line. (AU)

• Processo FAPESP: 16/03444-5 - Desenvolvimento de formulação farmacêutica cicatrizante contendo bromelina extraída de resíduos industriais
• Beneficiário: Priscila Gava Mazzola
• Modalidade de apoio: Auxílio à Pesquisa - Regular