Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats

Autor(es)

Mendes, Leonardo O. ^[1] ; Castilho, Anthony C. S. ^[1] ; Pinho, Cristiane F. ^[2] ; Goncalvez, Bianca F. ^[2] ; Razza, Eduardo M. ^[3] ; Chuffa, Luiz Gustavo A. ^[4] ; Anselmo-Franci, Janete A. ^[5] ; Scarano, Wellerson R. ^[2] ; Martinez, Francisco E. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Western Sao Paulo, Grad Program Anim Sci, Campus 2, RodoviaRaposo Tavares, Km 572, BR-19067175 Presidente Prudente, SP - Brazil

^[2] Sao Paulo State Univ, Dept Morphol, Inst Biosci, Botucatu Campus, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ, Dept Pharmacol, Inst Biosci, Botucatu Campus, Botucatu, SP - Brazil

^[4] Sao Paulo State Univ, Dept Anat, Inst Biosci, Botucatu Campus, Botucatu, SP - Brazil

^[5] Univ Sao Paulo, Dept Morphol Stomatol & Physiol, RibeiraoPreto Campus, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 42, n. 9, p. 1200-1211, SEP 2018.
• Citações Web of Science: 0

Resumo

Testosterone is often recommended in the treatment of several aging-related conditions. However, there are still questions about the consequences of this therapy in terms of hormonal and inflammatory parameters that are crucial for prostate homeostasis. Thus, we investigate if the testosterone therapy (TT) modulates the hormone receptors and inflammatory cytokines in the ventral prostate of adult rats. Wistar rats aging 150 days were divided into two experimental groups (n=10/group): T: received subcutaneous injections of testosterone cypionate (5mg/kg body weight) diluted in corn oil every other day for 4 weeks; and C: received corn oil as vehicle. Animals were euthanized at 180 days old by decapitation. Blood was collected to obtain hormone and cytokines concentrations. The ventral prostate was dissected and processed for light microscope and molecular analyses. Relative ventral prostate weight and epithelial compartment were increased after TT. The number of intact and degranulated mast cells was reduced in the T group. Plasma testosterone, DHT and intraprostatic testosterone concentrations were higher in the T group. TT leads to an increase in cell proliferation and up-regulation of AR, ER, PAR-4, and NRF2. Importantly, plasma concentration and tissue expression of IL-10 and TNF- were higher after TT. In summary, these results indicate that TT can regulate inflammatory response, with impacts in cytokines and mast cell population, and modulates steroids receptors, important parameters for prostatic homeostasis. (AU)

• Processo FAPESP: 11/05936-9 - Terapia de reposição hormonal na próstata de ratos UChB: expressão de proteínas inflamatórias e de receptores hormonais
• Beneficiário: Francisco Eduardo Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54606-1 - Caracterizacao do processo inflamatorio e influencia da terapia de reposicao hormonal no lobo ventral da prostata de ratos bebedores voluntarios de etanol a 1o%(uchb)
• Beneficiário: Leonardo de Oliveira Mendes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/00917-9 - Terapia de reposição hormonal no lobo ventral da próstata de ratos UChB: efeitos sobre os receptores estrogênicos (er alfa e er beta) e na via de sinalização do TGF-beta 1
• Beneficiário: Leonardo de Oliveira Mendes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado