| Texto completo | |
| Autor(es): |
Vicente, Acacia Ferreira
[1, 2, 3]
;
Girault, Guillaume
[1, 2]
;
Corde, Yannick
[1, 2, 4]
;
Mioni, Mateus Souza Ribeiro
[3]
;
Keid, Lara Borges
[5]
;
Jay, Maryne
[1, 2, 6, 7]
;
Megid, Jane
[3]
;
Mick, Virginie
[1, 2]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Paris Est Univ Anses, Hlth Anim Lab, EU OIE FAO, Maisons Alfort - France
[2] Paris Est Univ Anses, Hlth Anim Lab, Natl Reference Lab Anim Brucellosis, Maisons Alfort - France
[3] UNESP, FMVZ, Dept Vet Hyg & Publ Hlth, Mol Biol Lab, Botucatu, SP - Brazil
[4] INRA, Physiol Reprod & Behav Joint Res Unit, Val Loire Ctr, Nouzilly - France
[5] Univ Anim Sci & Food Engn, USP, Dept Vet Med, Pirassununga - Brazil
[6] Anses, Lab Lyon, UMR Mycoplasmoses Ruminants, Lyon - France
[7] Univ Lyon, VetAgro Sup, UMR Mycoplasmoses Ruminants, Marcy Letoile - France
Número total de Afiliações: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC Genomics; v. 19, AUG 28 2018. |
| Citações Web of Science: | 1 |
| Resumo | |
Background: Canine brucellosis, due to Brucella canis, is a worldwide zoonosis that remains endemic in South America, including Brazil. Implementation of powerful whole-genome sequencing approaches allowed exploring the Brucella genus considered as monomorphic, with, to date, more than 500 genomes available in public databases. Nevertheless, with under-representation of B. canis genomes -only twenty complete or draft genomes-, lack of knowledge about this species is still considerable. This report describes a comparative genomics-based phylogeographic investigation of 53 B. canis strains, including 28 isolates paired-end sequenced in this work. esults: Obtained results allow identifying a SNP panel species-specific to B. canis of 1086 nucleotides. In addition, high-resolution analyses assess the epidemiological relationship between worldwide isolates. Our findings show worldwide strains are distributed among 2 distinct lineages. One of them seems to be specific to South American strains, including Brazil. B. canis South American strains may be identified by a SNP panel of 15 nucleotides, whereas a 22 SNP panel is sufficient to define contamination origin from Brazil. These results lead to the proposal of a possible spread route for dog brucellosis through South America. Additionally, whole-genome analyses highlight the remarkable genomic stability of B. canis strains over time and the sustainability of the infection in Sao Paulo over 12 year-period. Conclusions: Significant increase of B. canis genomes available in public databases provides new insights into B. canis infection in South America, including Brazil, as well as in the world, and also offers new perspectives for the Brucella genus largo sensu. (AU) | |
| Processo FAPESP: | 14/00889-0 - Brucelose canina: avaliação da diversidade genética e desenvolvimento de novas ferramentas de tipagem molecular para Brucella canis |
| Beneficiário: | Acácia Ferreira Vicente |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |