Differentially expressed proteins in positive versus negative HNSCC lymph nodes

Autor(es):Mostrar menos -

Vidotto, Alessandra ^[1] ; Polachini, Giovana M. ^[1] ; de Paula-Silva, Marina ^[2] ; Oliani, Sonia M. ^[2] ; Henrique, Tiago ^[1] ; Lopez, Rossana V. M. ^[3] ; Cury, Patricia M. ^[4] ; Nunes, Fabio D. ^[5] ; Gois-Filho, Jose F. ^[6] ; de Carvalho, Marcos B. ^[7] ; Leopoldino, Andreia M. ^[8] ; Tajara, Eloiza H. ^{[1, 9]}

Afiliação do(s) autor(es)

^[1] Fac Med FAMERP, Dept Biol Mol, Ave Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas IBILCE, Dept Biol, R Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Inst Canc Sao Paulo Octavio Frias de Oliveira ICE, Ave Dr Arnaldo 251, BR-01246000 Sao Paulo, SP - Brazil

^[4] Fac Ceres Faceres, Ave Anisio Haddad 6751, BR-15090305 Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Sao Paulo, Dept Estomatol, Fac Odontol, Ave Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

^[6] Inst Canc Arnaldo Vieira de Carvalho, R Dr Cesario Mota Jr 112, BR-01221020 Sao Paulo, SP - Brazil

^[7] Hosp Heliopolis, Dept Cirurgia Cabeca & Pescoco, R Conego Xavier 276, BR-04231030 Sao Paulo, SP - Brazil

^[8] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[9] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, R Matao 321, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC MEDICAL GENOMICS; v. 11, AUG 29 2018.
• Citações Web of Science: 1

Resumo

Background: Lymph node metastasis is one of the most important prognostic factors in head and neck squamous cell carcinomas (HNSCCs) and critical for delineating their treatment. However, clinical and histological criteria for the diagnosis of nodal status remain limited. In the present study, we aimed to characterize the proteomic profile of lymph node metastasis from HNSCC patients. Methods: In the present study, we used one-and two-dimensional electrophoresis and mass spectrometry analysis to characterize the proteomic profile of lymph node metastasis from HNSCC. Results: Comparison of metastatic and non-metastatic lymph nodes showed 52 differentially expressed proteins associated with neoplastic development and progression. The results reinforced the idea that tumors from different anatomical subsites have dissimilar behaviors, which may be influenced by micro-environmental factor including the lymphatic network. The expression pattern of heat shock proteins and glycolytic enzymes also suggested an effect of the lymph node environment in controlling tumor growth or in metabolic reprogramming of the metastatic cell. Our study, for the first time, provided direct evidence of annexin A1 overexpression in lymph node metastasis of head and neck cancer, adding information that may be useful for diagnosing aggressive disease. Conclusions: In brief, this study contributed to our understanding of the metastatic phenotype of HNSCC and provided potential targets for diagnostic in this group of carcinomas. (AU)

• Processo FAPESP: 04/14846-0 - Rede de proteoma do estado de São Paulo
• Beneficiário: Fabio Cesar Gozzo
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA
• Processo FAPESP: 04/12054-9 - Busca de marcadores de agressividade em tumores de cabeça e pescoço
• Beneficiário: Eloiza Helena Tajara da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático