The role of vitamin C in the gene expression of oxidative stress markers in fibroblasts from burn patients

Autor(es)

Bonucci, Jessica ^[1] ; Gragnani, Alfredo ^[2] ; Trincado, Marcelo Moraes ^[3] ; Vincentin, Victor ^[3] ; Alves Correa, Silvana Aparecida ^[2] ; Ferreira, Lydia Masako ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Surg, Div Plast Surg, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Surg, Div Plast Surg, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Div Plast Surg, Researcher CNPq 1A, Director Med CAPES 3, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 33, n. 8, p. 703-712, AUG 2018.
• Citações Web of Science: 1

Resumo

Abstract Purpose: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. Methods: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis using quantitative polymerase chain reaction array was performed for comparisons between groups. Results: The comparison revealed 10 upregulated genes as follows: arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol reductase (DHCR24), dual oxidase 1 (DUOX1), glutathione peroxidase 2 (GPX2), glutathione peroxidase 5 (GPX5), microsomal glutathione S-transferase 3 (MGST3), peroxiredoxin 4 (PRDX4), phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (P-REX1), prostaglandin-endoperoxide synthase 1 (PTGS1), and ring finger protein 7 (RNF7). Conclusion: Cultured fibroblasts obtained from burn patients and treated with vitamin C resulted in 10 differentially expressed genes, all overexpressed, with DUOX1, GPX5, GPX2 and PTGS1 being of most interest. (AU)

• Processo FAPESP: 13/10905-0 - Fator de crescimento de queratinócitos na expressão gênica do sistema imunológico e da cicatrização em células-tronco da pele de pacientes queimados
• Beneficiário: Alfredo Gragnani Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular