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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The role of vitamin C in the gene expression of oxidative stress markers in fibroblasts from burn patients

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Author(s):
Bonucci, Jessica [1] ; Gragnani, Alfredo [2] ; Trincado, Marcelo Moraes [3] ; Vincentin, Victor [3] ; Alves Correa, Silvana Aparecida [2] ; Ferreira, Lydia Masako [4]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Surg, Div Plast Surg, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Surg, Div Plast Surg, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo, Div Plast Surg, Researcher CNPq 1A, Director Med CAPES 3, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Acta Cirurgica Brasileira; v. 33, n. 8, p. 703-712, AUG 2018.
Web of Science Citations: 1
Abstract

Abstract Purpose: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. Methods: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis using quantitative polymerase chain reaction array was performed for comparisons between groups. Results: The comparison revealed 10 upregulated genes as follows: arachidonate 12-lipoxygenase (ALOX12), 24-dehydrocholesterol reductase (DHCR24), dual oxidase 1 (DUOX1), glutathione peroxidase 2 (GPX2), glutathione peroxidase 5 (GPX5), microsomal glutathione S-transferase 3 (MGST3), peroxiredoxin 4 (PRDX4), phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 (P-REX1), prostaglandin-endoperoxide synthase 1 (PTGS1), and ring finger protein 7 (RNF7). Conclusion: Cultured fibroblasts obtained from burn patients and treated with vitamin C resulted in 10 differentially expressed genes, all overexpressed, with DUOX1, GPX5, GPX2 and PTGS1 being of most interest. (AU)

FAPESP's process: 13/10905-0 - Keratinocyte growth factor in gene expression of immune system and wound healing in stem cells of the skin from burned patients
Grantee:Alfredo Gragnani Filho
Support Opportunities: Regular Research Grants