| Texto completo | |
| Autor(es): |
Campos, Rafael
[1]
;
Oliveira Justo, Alberto Fernando
[1]
;
Monica, Fabiola Z.
[1]
;
Cogo, Jose Carlos
[2]
;
Moreno, Ronilson Agnaldo
[1]
;
de Souza, Valeria Barbosa
[1]
;
Schenka, Andre Almeida
[1]
;
De Nucci, Gilberto
[1, 2]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, UNICAMP, Dept Pharmacol, Fac Med Sci, Campinas, SP - Brazil
[2] Brazil Univ, Fac Biomed Engn, Itaquera - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 13, n. 9 SEP 10 2018. |
| Citações Web of Science: | 1 |
| Resumo | |
Endothelium is the main source of catecholamine release in the electrical-field stimulation (EFS)-induced aortic contractions of the non- venomous snake Panterophis guttatus. However, adrenergic vasomotor control in venomous snakes such as Crotalus durissus terrificus and Bothrops jararaca has not yet been investigated. Crotalus and Bothrops aortic rings were mounted in an organ bath system. EFS-induced aortae contractions were performed in the presence and absence of guanethidine (30 mu M), phentolamine (10 mu M) or tetrodotoxin (1 mu M). Frequency-induced contractions were also performed in aortae with endothelium removed. Immunohistochemical localization of both tyrosine hydroxylase (TH) and S-100 protein in snake aortic rings and brains, as well as in human tissue (paraganglioma tumour) were carried out. EFS (4 to 16 Hz) induced frequency-dependent aortic contractions in both Crotalus and Bothrops. The EFS-induced contractions were significantly reduced in the presence of either guanethidine or phentolamine in both snakes (p<0.05), whereas tetrodotoxin had no effect in either. Removal of the endothelium abolished the EFS-induced contractions in both snakes aortae (p<0.05). Immunohistochemistry revealed TH localization in endothelium of both snake aortae and human vessels. Nerve fibers were not observed in either snake aortae. In contrast, both TH and S100 protein were observed in snake brains and human tissue. Vascular endothelium is the main source of catecholamine release in EFS-induced contractions in Crotalus and Bothrops aortae. Human endothelial cells also expressed TH, indicating that endothelium- derived catecholamines possibly occur in mammalian vessels. (AU) | |
| Processo FAPESP: | 11/11828-4 - Identificação e caracterização farmacológica, eletrofisiológica e morfológica de novo canal de sódio TTX-resistente acoplado à musculatura lisa de corpo cavernoso de cobra |
| Beneficiário: | Gilberto de Nucci |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |