Expression of Hbs, Kirre, and Rst during Drosophila ovarian development

Autor(es)

Valer, Felipe Berti ^[1] ; Rosa Machado, Maiaro Cabral ^[1] ; Patricio Silva-Junior, Rui Milton ^[1] ; Pinheiro Ramos, Ricardo Guelerman ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cellular & Mol Biol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENESIS; v. 56, n. 9 SEP 2018.
• Citações Web of Science: 2

Resumo

The Irre cell-recognition module (IRM) is a group of evolutionarily conserved and structurally related transmembrane glycoproteins of the immunoglobulin superfamily. In Drosophila melanogaster, it comprises the products of the genes roughest (rst; also known as irreC-rst), kin-of-irre (kirre; also known as duf), sticks-and-stones (sns), and hibris (hbs). In this model organism, the behavior of this group of proteins as a partly redundant functional unit mediating selective cell recognition was demonstrated in a variety of developmental contexts, but their possible involvement in ovarian development and oogenesis has not been investigated, notwithstanding the fact that some rst mutant alleles are also female sterile. Here, we show that IRM genes are dynamically and, to some extent, coordinately transcribed in both pupal and adult ovaries. Additionally, the spatial distribution of Hbs, Kirre, and Rst proteins indicates that they perform cooperative, although largely nonredundant, functions. Finally, phenotypical characterization of three different female sterile rst alleles uncovered two temporally separated and functionally distinct requirements for this locus in ovarian development: one in pupa, essential for the organization of peritoneal and epithelial sheaths that maintain the structural integrity of the adult organ and another, in mature ovarioles, needed for the progression of oogenesis beyond stage 10. (AU)

• Processo FAPESP: 15/16896-9 - Estudo da família IRM de moléculas de adesão celular durante o desenvolvimento animal: funções, regulação e conservação evolutiva
• Beneficiário: Ricardo Guelerman Pinheiro Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular