| Texto completo | |
| Autor(es): |
de Faria, Amanda N.
[1, 2]
;
Cruz, Marcos A. E.
[1]
;
Ruiz, Gilia C. M.
[1]
;
Zancanela, Daniela C.
[1]
;
Ciancaglini, Pietro
[1]
;
Ramos, Ana P.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-3900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-3900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 106, n. 7, p. 2524-2534, OCT 2018. |
| Citações Web of Science: | 5 |
| Resumo | |
Calcium phosphates (CaPs) are biomaterials widely used in tissue regeneration with outstanding biological performance. Although the tremendous improvements achieved in CaP's materials research over the years, their interaction with physiological environments still need to be fully understood. The aim of this study is to explore a biomimetic Langmuir-Blodgett (LB) membrane to template the growth of hydroxyapatite (HAp) coatings on Ti surfaces and the ability of these coatings in inducing biomineralization by osteoblasts cultured in vitro. Changing the phospholipids (i.e., dihexadecyl phosphate (DHP) or octadecylphosphonic acid (OPA)), we also tuned the surface Ca2+ concentration. This structural feature gave rise to different LB-hybrid surfaces where the concentration of Ca2+ in the OPA/HAp was higher than the concentration of Ca2+ in DHP/HAp coating. The higher Ca2+ amount on OPA/HAp coatings, allied to the physical-chemical features, lead to different responses on osteoblasts, stimulating or inhibiting the natural biomineralization. The OPA/HAp coating caused a delay in the osteoblast proliferation as indicated by the decrease in the cell viability at the 7th culture day. Improved cell differentiation triggered by the DHP/HAp coating resulted in higher osteoblast biomineralization. The present data underscore that besides both coatings being composed by HAp, the final interfacial composition and physical-chemical properties influence differently the osteoblast behavior. Although the best osteoblast's viability was found to OPA/HAp, our dataset attested that DHP/HAp induced mineralization more effectively than that. This unexpected finding highlight the importance of deeply understanding the biomaterial interface and suggest a promising approach to the design of biofunctional LB-based coatings with tunable properties. (C) 2018 Wiley Periodicals, Inc. (AU) | |
| Processo FAPESP: | 14/11941-3 - Colágeno e annexina são responsáveis pelo controle do processo de biomineralização? |
| Beneficiário: | Pietro Ciancaglini |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/08868-0 - Consolidação do Centro de Microscopia Funcional do Campus da USP/ Ribeirão Preto |
| Beneficiário: | Roy Edward Larson |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |