ent-Copalic acid antibacterial and anti-biofilm properties against Actinomyces naeslundii and Peptostreptococcus anaerobius

Autor(es)

Mendes de Souza, Maria Gorete ^[1] ; Leandro, Luis Fernando ^[1] ; Moraes, Thais da Silva ^[1] ; Abrao, Fariza ^[1] ; Sola Veneziani, Rodrigo Cassio ^[2] ; Ambrosio, Sergio Ricardo ^[2] ; Gomes Martins, Carlos Henrique ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca UNIFRAN, Lab Res Appl Microbiol, Franca, SP - Brazil

^[2] Univ Franca UNIFRAN, Nucleus Res Sci & Technol, Franca, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anaerobe; v. 52, p. 43-49, AUG 2018.
• Citações Web of Science: 2

Resumo

Diterpenes are an important class of plant metabolites that can be used in the search for new antibacterial agents. ent-Copalic acid (CA), the major diterpene in Copaifera species exudates, displays several pharmacological properties. This study evaluates the CA antibacterial potential against the anaerobic bacteria Peptostreptococcus anaerobius and Actinomyces naeslundii. Antimicrobial assays included time-kill and biofilm inhibition and eradication assays. Time-kill assays conducted for CA concentrations between 6.25 and 12.5 mu g/mL evidenced bactericidal activity within 72 h. CA combined with chlorhexidine dihydrochloride (CHD) exhibited bactericidal action against P. anaerobius within 6 h of incubation. As for A. naeslundii, the same combination reduced the number of microorganisms by over 3 log10 at 24 h and exerted a bactericidal effect at 48 h of incubation. CA at 500 and 2000 mu g/mL inhibited P. anaerobius and A. naeslundii biofilm formation by at least 50%, respectively. CA at 62.5 and 1.000 mu g/mL eradicated 99.9% of pre-formed P. anaerobius and A. naeslundii biofilms, respectively. These results indicated that CA presents in vitro antibacterial activity and is a potential biofilm inhibitory agent. This diterpene may play an important role in the search for novel sources of agents that can act against anaerobic bacteria. (C) 2018 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/25237-0 - Diterpenos do tipo caurano: atividade antibacteriana frente a bactérias anaeróbias e análise proteômica
• Beneficiário: Carlos Henrique Gomes Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular