Interactions of ethanol and caffeine on apoptosis in the rat cerebellum (voluntary ethanol consumers)

Autor(es)

Martinez, Marcelo ^[1] ; Rossetto, Isabela M. U. ^[1] ; Lizarte Neto, Fermino Sanches ^[2] ; Tirapelli, Luiz F. ^[2] ; Tirapelli, Daniela P. C. ^[2] ; Chuffa, Luiz Gustavo A. ^[3] ; Cagnon, Valeria H. A. ^[4] ; Martinez, Francisco E. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Morphol & Pathol, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Dept Surg & Anat, Ribeirao Preto, SP - Brazil

^[3] State Univ Sao Paulo, Dept Anat, Botucatu, SP - Brazil

^[4] Univ Estadual Campinas, Dept Anat Cellular Biol Physiol & Biophys, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 42, n. 11, p. 1575-1583, NOV 2018.
• Citações Web of Science: 3

Resumo

Ethanol alters motricity, learning, cognition, and cellular metabolism in the cerebellum. The combination of ethanol with caffeine by consuming energy drinks is becoming increasingly popular among young people. We analyzed the use of ethanol and caffeine on apoptosis in the cerebellum of UChB rats. The adult rats were divided into three groups (n=14/group): UChB group: rats fed with 1:10 (v/v) ethanol ad libitum (free choice for water or ethanol) drinking from >1.9mL of ethanol/kg body weight/day, Control group and UChB/caffeine group (free choice for water or ethanol+caffeine 300mg/L). The treatments occurred from Day 100 till Day 150, totalizing 50 days of ethanol/caffeine ingestion. Cerebellar sections were subjected to immunohistochemistry and gene expression for Real Time-PCR (RT-PCR) for Caspase-3, XIAP, and insulin-like growth factor 1-receptor (IGF-1R). The results showed a significant increase in the gene expression of Caspase-3 and XIAP in UChB group. On the other hand, the animals of the UChB/caffeine group showed similar results to the Controls. Regarding IGFR-1, there was greater expression in the UChB groups with strong labeling in Purkinje cells. Ethanol produces neuronal and glial neurodegeneration on the cerebellum of UChB rats. The simultaneous ingestion of ethanol and caffeine reversed the ethanol damages acting caffeine with a neuroprotective effect. (AU)

• Processo FAPESP: 13/11095-2 - Efeitos do consumo simultâneo de etanol e cafeína na apoptose no cerebelo de ratos UChB (consumidores voluntários de etanol): microRNAs e expressões gênicas
• Beneficiário: Marcelo Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/06240-6 - Efeitos do consumo simultâneo de etanol e cafeína no apoptose no cerebelo de ratos UChB (consumidores voluntários de etanol): Análise proteica da Caspase 3, Xiap e IGFR-1
• Beneficiário: Isabela Maria Urra Rossetto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica