Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interactions of ethanol and caffeine on apoptosis in the rat cerebellum (voluntary ethanol consumers)

Full text
Author(s):
Martinez, Marcelo [1] ; Rossetto, Isabela M. U. [1] ; Lizarte Neto, Fermino Sanches [2] ; Tirapelli, Luiz F. [2] ; Tirapelli, Daniela P. C. [2] ; Chuffa, Luiz Gustavo A. [3] ; Cagnon, Valeria H. A. [4] ; Martinez, Francisco E. [3]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Morphol & Pathol, Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Dept Surg & Anat, Ribeirao Preto, SP - Brazil
[3] State Univ Sao Paulo, Dept Anat, Botucatu, SP - Brazil
[4] Univ Estadual Campinas, Dept Anat Cellular Biol Physiol & Biophys, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Cell Biology International; v. 42, n. 11, p. 1575-1583, NOV 2018.
Web of Science Citations: 3
Abstract

Ethanol alters motricity, learning, cognition, and cellular metabolism in the cerebellum. The combination of ethanol with caffeine by consuming energy drinks is becoming increasingly popular among young people. We analyzed the use of ethanol and caffeine on apoptosis in the cerebellum of UChB rats. The adult rats were divided into three groups (n=14/group): UChB group: rats fed with 1:10 (v/v) ethanol ad libitum (free choice for water or ethanol) drinking from >1.9mL of ethanol/kg body weight/day, Control group and UChB/caffeine group (free choice for water or ethanol+caffeine 300mg/L). The treatments occurred from Day 100 till Day 150, totalizing 50 days of ethanol/caffeine ingestion. Cerebellar sections were subjected to immunohistochemistry and gene expression for Real Time-PCR (RT-PCR) for Caspase-3, XIAP, and insulin-like growth factor 1-receptor (IGF-1R). The results showed a significant increase in the gene expression of Caspase-3 and XIAP in UChB group. On the other hand, the animals of the UChB/caffeine group showed similar results to the Controls. Regarding IGFR-1, there was greater expression in the UChB groups with strong labeling in Purkinje cells. Ethanol produces neuronal and glial neurodegeneration on the cerebellum of UChB rats. The simultaneous ingestion of ethanol and caffeine reversed the ethanol damages acting caffeine with a neuroprotective effect. (AU)

FAPESP's process: 13/11095-2 - Simultaneous effects of ethanol and caffeine consumption in apoptosis of UChB rat's cerebellum (voluntary consumers of ethanol): microRNAs and gene expressions
Grantee:Marcelo Martinez
Support Opportunities: Regular Research Grants
FAPESP's process: 14/06240-6 - Effects of simultaneous consumption of ethanol and caffeine on apoptosis in the cerebellum of UChB rats (voluntary ethanol consumers): Proteic analysis of Caspase 3, XIAP, and IGFR-1
Grantee:Isabela Maria Urra Rossetto
Support Opportunities: Scholarships in Brazil - Scientific Initiation