Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of Glutaraldehyde Cross-Linking Modes on the Recyclability of Immobilized Lipase B from Candida antarctica for Transesterification of Soy Bean Oil

Texto completo
Autor(es):
Modenez, Iago A. [1] ; Sastre, Diego E. [2] ; Moraes, Fernando C. [3] ; Marques Netto, Caterina G. C. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Lab Metaloenzimas & Biomimet, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Dept Fis & Ciencias Interdisciplinares, Inst Fis, BR-13563120 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Lab Analit Bioanalit Biossensores Eletroanalit &, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Molecules; v. 23, n. 9 SEP 2018.
Citações Web of Science: 2
Resumo

Lipase B from Candida antarctica (CAL-B) is largely employed as a biocatalyst for hydrolysis, esterification, and transesterification reactions. CAL-B is a good model enzyme to study factors affecting the enzymatic structure, activity and/or stability after an immobilization process. In this study, we analyzed the immobilization of CAL-B enzyme on different magnetic nanoparticles, synthesized by the coprecipitation method inside inverse micelles made of zwitterionic surfactants, with distinct carbon chain length: 4 (ImS4), 10 (ImS10) and 18 (ImS18) carbons. Magnetic nanoparticles ImS4 and ImS10 were shown to cross-link to CAL-B enzyme via a Michael-type addition, whereas particles with ImS18 were bond via pyridine formation after glutaraldehyde cross-coupling. Interestingly, the Michael-type cross-linking generated less stable immobilized CAL-B, revealing the influence of a cross-linking mode on the resulting biocatalyst behavior. Curiously, a direct correlation between nanoparticle agglomerate sizes and CAL-B enzyme reuse stability was observed. Moreover, free CAL-B enzyme was not able to catalyze transesterification due to the high methanol concentration; however, the immobilized CAL-B enzyme reached yields from 79.7 to 90% at the same conditions. In addition, the transesterification of lipids isolated from oleaginous yeasts achieved 89% yield, which confirmed the potential of immobilized CAL-B enzyme in microbial production of biodiesel. (AU)

Processo FAPESP: 16/01622-3 - Miméticos de urease: catálise e compreensão mecanística
Beneficiário:Caterina Gruenwaldt Cunha Marques Netto
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/13411-1 - Estudo de aciltransferases necessárias para a síntese de fosfolipídeos de membrana de bactérias Gram-positivas: um alvo atraente para a descoberta de drogas anti-bacterianas
Beneficiário:Diego Emiliano Sastre
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado