Influence of Glutaraldehyde Cross-Linking Modes on the Recyclability of Immobilized Lipase B from Candida antarctica for Transesterification of Soy Bean Oil

Author(s)

Modenez, Iago A. ^[1] ; Sastre, Diego E. ^[2] ; Moraes, Fernando C. ^[3] ; Marques Netto, Caterina G. C. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Lab Metaloenzimas & Biomimet, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Dept Fis & Ciencias Interdisciplinares, Inst Fis, BR-13563120 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Lab Analit Bioanalit Biossensores Eletroanalit &, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Molecules; v. 23, n. 9 SEP 2018.
• Web of Science Citations: 2

Abstract

Lipase B from Candida antarctica (CAL-B) is largely employed as a biocatalyst for hydrolysis, esterification, and transesterification reactions. CAL-B is a good model enzyme to study factors affecting the enzymatic structure, activity and/or stability after an immobilization process. In this study, we analyzed the immobilization of CAL-B enzyme on different magnetic nanoparticles, synthesized by the coprecipitation method inside inverse micelles made of zwitterionic surfactants, with distinct carbon chain length: 4 (ImS4), 10 (ImS10) and 18 (ImS18) carbons. Magnetic nanoparticles ImS4 and ImS10 were shown to cross-link to CAL-B enzyme via a Michael-type addition, whereas particles with ImS18 were bond via pyridine formation after glutaraldehyde cross-coupling. Interestingly, the Michael-type cross-linking generated less stable immobilized CAL-B, revealing the influence of a cross-linking mode on the resulting biocatalyst behavior. Curiously, a direct correlation between nanoparticle agglomerate sizes and CAL-B enzyme reuse stability was observed. Moreover, free CAL-B enzyme was not able to catalyze transesterification due to the high methanol concentration; however, the immobilized CAL-B enzyme reached yields from 79.7 to 90% at the same conditions. In addition, the transesterification of lipids isolated from oleaginous yeasts achieved 89% yield, which confirmed the potential of immobilized CAL-B enzyme in microbial production of biodiesel. (AU)

• FAPESP's process: 16/01622-3 - Urease mimetics: catalysis and mechanism comprehension
• Grantee: Caterina Gruenwaldt Cunha Marques Netto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/13411-1 - Study of acyltransferases required for the synthesis of membrane phospholipids in Gram-positive bacteria: an attractive target for antibacterial drug discovery
• Grantee: Diego Emiliano Sastre
• Support Opportunities: Scholarships in Brazil - Post-Doctoral