Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of Glutaraldehyde Cross-Linking Modes on the Recyclability of Immobilized Lipase B from Candida antarctica for Transesterification of Soy Bean Oil

Full text
Author(s):
Modenez, Iago A. [1] ; Sastre, Diego E. [2] ; Moraes, Fernando C. [3] ; Marques Netto, Caterina G. C. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Lab Metaloenzimas & Biomimet, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Dept Fis & Ciencias Interdisciplinares, Inst Fis, BR-13563120 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Lab Analit Bioanalit Biossensores Eletroanalit &, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 23, n. 9 SEP 2018.
Web of Science Citations: 2
Abstract

Lipase B from Candida antarctica (CAL-B) is largely employed as a biocatalyst for hydrolysis, esterification, and transesterification reactions. CAL-B is a good model enzyme to study factors affecting the enzymatic structure, activity and/or stability after an immobilization process. In this study, we analyzed the immobilization of CAL-B enzyme on different magnetic nanoparticles, synthesized by the coprecipitation method inside inverse micelles made of zwitterionic surfactants, with distinct carbon chain length: 4 (ImS4), 10 (ImS10) and 18 (ImS18) carbons. Magnetic nanoparticles ImS4 and ImS10 were shown to cross-link to CAL-B enzyme via a Michael-type addition, whereas particles with ImS18 were bond via pyridine formation after glutaraldehyde cross-coupling. Interestingly, the Michael-type cross-linking generated less stable immobilized CAL-B, revealing the influence of a cross-linking mode on the resulting biocatalyst behavior. Curiously, a direct correlation between nanoparticle agglomerate sizes and CAL-B enzyme reuse stability was observed. Moreover, free CAL-B enzyme was not able to catalyze transesterification due to the high methanol concentration; however, the immobilized CAL-B enzyme reached yields from 79.7 to 90% at the same conditions. In addition, the transesterification of lipids isolated from oleaginous yeasts achieved 89% yield, which confirmed the potential of immobilized CAL-B enzyme in microbial production of biodiesel. (AU)

FAPESP's process: 14/13411-1 - Study of acyltransferases required for the synthesis of membrane phospholipids in Gram-positive bacteria: an attractive target for antibacterial drug discovery
Grantee:Diego Emiliano Sastre
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/01622-3 - Urease mimetics: catalysis and mechanism comprehension
Grantee:Caterina Gruenwaldt Cunha Marques Netto
Support type: Regular Research Grants