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Immobilization and stabilization of lipases from Candida antarctida: fraction b and its application in biocatalysis

Grant number: 13/00530-0
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): September 01, 2013
Effective date (End): February 28, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal researcher:Mauricio Boscolo
Grantee:José Carlos Quilles Junior
Supervisor abroad: José M. Guisan
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
Research place: Universidad Autónoma de Madrid (UAM), Spain  
Associated to the scholarship:12/09054-3 - Immobilization of lipases on agarose and chitosan and application in biocatalysis., BP.MS


The project stage of research abroad aims to improve work techniques immobilization and stabilization of lipases and their application in biocatalysis of reactions of hydrolysis and transesterification of vegetable oil. Lipases from Candida antarctica - fraction B will be immobilized by different methods of immobilization (covalent bonding and hydrophobic interaction) and its stability will be studied in ethanol and its thermostability. The immobilized enzymes are applied in reactions of racemic mixtures to try to distinguish the enantiomer of interest, on hydrolysis of fish oil to produce free fatty acids and ethanolysis reaction on vegetable oil for the synthesis of ethyl biodiesel. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BORGES, JANAINA PIRES; QUILLES JUNIOR, JOSE CARLOS; MORENO-PEREZ, SONIA; FERNANDEZ-LORENTE, GLORIA; BOSCOLO, MAURICIO; GOMES, ELENI; DA SILVA, ROBERTO; BOCCHINI, DANIELA ALONSO; GUISAN, JOSE MANUEL. Ethyl esters production catalyzed by immobilized lipases is influenced byn-hexaneandter-amyl alcohol as organic solvents. Bioprocess and Biosystems Engineering, JUN 2020. Web of Science Citations: 0.
QUILLES JUNIOR, JOSE CARLOS; FERRAREZI, ANA LUCIA; BORGES, JANAINA PIRES; BRITO, RAFAELA RODRIGUES; GOMES, ELENI; DA SILVA, ROBERTO; MANUEL GUISAN, JOSE; BOSCOLO, MAURICIO. Hydrophobic adsorption in ionic medium improves the catalytic properties of lipases applied in the triacylglycerol hydrolysis by synergism. Bioprocess and Biosystems Engineering, v. 39, n. 12, p. 1933-1943, DEC 2016. Web of Science Citations: 4.
QUILLES, J. C. J.; BRITO, R. R.; BORGES, J. P.; ARAGON, CC.; FERNANDEZ-LORENTED, G.; BOCCHINI-MARTINS, D. A.; GOMES, E.; DA SILVA, R.; BOSCOLO, M.; GUISAN, J. M. Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents. Biochemical Engineering Journal, v. 93, p. 274-280, JAN 15 2015. Web of Science Citations: 23.

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