Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves

Autor(es)

Santana, Andre M. ^[1] ; Silva, Daniela G. ^[1] ; Thomas, Funmilola C. ^[2] ; Bernardes, Priscila A. ^[1] ; Pizauro, Lucas J. L. ^[1] ; Santana, Clarissa H. ^[1] ; Burchmore, Richard J. S. ^[3] ; Eckersall, Peter D. ^[4] ; Fagliari, Jose J. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ FCAV UNESP, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabtil, SP - Brazil

^[2] Fed Univ Agr, Coll Vet Med, Dept Vet Physiol & Pharmacol, Abeokuta - Nigeria

^[3] Univ Glasgow, Glasgow Poly Facil, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow, Lanark - Scotland

^[4] Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Coll Med Vet & Life Sci, Glasgow, Lanark - Scotland

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 203, p. 30-39, SEP 2018.
• Citações Web of Science: 0

Resumo

The study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 10(8) CFU of S. Dublin (INF). Clinical evaluation was performed, rectal swabs to detect S. Dublin strains were collected and venous blood was sampled before and throughout seven days after inoculation. The APP fractions beta-haptoglobin, alpha-haptoglobin, ceruloplasmin and transferrin were analyzed by 1-D and 2-D electrophoresis. Proteins were identified using LC/ESI-MS/MS and NCBI database. Plasma fibrinogen, serum iron and serum haptoglobin concentrations were measured. The inoculation of 10(8) CFU of S. Dublin was effective in inducing clinical signs of Salmonellosis, such as hyperthermia and diarrhea. 1-DE showed that beta and alpha-haptoglobin increased 204% (p = 0.008) and 184% (p = 0.022) 48 h after inoculation (HAI), respectively, with highest concentrations 120 HAI (498% increased, p = 0.012; 431% increased, p = 0.011) and 168 HAI (492% increased, p = 0.019; 523% increased, p = 0.028). 2-DE showed that the expression of two spots, identified as beta-haptoglobin, were increased 693% (p = 0.0006) and 580% (p = 0.0003) 168 HAI, respectively, while one spot, identified as alpha-haptoglobin, increased 714% (p = 0.040). Haptoglobin concentrations increased 1339% (p < 0.0001) 168 HAI. 1-DE showed that ceruloplasmin increased 42% (p = 0.034) 48 HAI, with highest concentration 120 HAI (133% increased, p = 0.022). 2-DE showed that the expression of two spots, identified as ceruloplasmin, were increased 218% (p = 0.0153) and 85% (p = 0.0143) 168 HAI, respectively. Fibrinogen increased 78% (p = 0.012) 96 HAI, with highest concentration 120 HAI (increased 114%, p = 0.002). Iron decreased 33% 24 HAI (p = 0.015) and 37% 72 HAI (p = 0.029), and began to be restored 96 HAI. 1-DE showed that transferrin decreased 23% 120 HAI (p = 0.047), and that values were restored 168 HAI. 2-DE showed that expression patterns of transferrin comparing Oh and 168 HAI were similar, evidencing that values were restored 168 HAI. In conclusion, the inoculation of 10(8) CFU was effective in inducing hyperthermia and diahrrea. beta and alpha-haptoglobin, ceruloplasmin and fibrinogen worked as positive APP during the APR to S. Dublin infection and are potential biomarker candidates. Concentrations of iron and transferrin decreased during the infection, highlighting the fact that mechanisms for restricting iron availability are part of the APR triggered against S. Dublin infection in buffalo calves. (AU)

• Processo FAPESP: 09/05410-7 - Avaliação clínica, hemograma e proteinograma sérico, com ênfase em proteínas de fase aguda e imunoglobulinas, de bezerros bubalinos experimentalmente infectados por Salmonella enterica subespécie enterica sorotipo Dublin
• Beneficiário: Priscila Arrigucci Bernardes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/12350-0 - Estudo clínico, laboratorial e terapêutico da infecção experimental de bezerros bubalinos por Salmonella dublin
• Beneficiário: José Jurandir Fagliari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/26498-5 - Estudo proteômico e de proteínas de fase aguda no soro sanguíneo e soro lácteo de búfalos e bovinos: mastite e salmonelose
• Beneficiário: André Marcos Santana
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 08/50388-7 - Infeccao experimental com salmonella dublin em bezerros bubalinos: estudo clinico, laboratorial e terapeutico.
• Beneficiário: André Marcos Santana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/05211-4 - Avaliação clínica, perfil bioquímico sérico e hemogasometria de bezerros bubalinos experimentalmente infectados por Salmonella enterica subespécie enterica sorotipo Dublin
• Beneficiário: Lucas José Luduverio Pizauro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/10094-0 - Perfil bioquímico da secreção láctea, inclusive proteínas de fase aguda e enzimas NAGase e LDH, como método auxiliar de diagnóstico e prognóstico de mastite subclínica e clínica em fêmeas bubalinas e bovinas
• Beneficiário: André Marcos Santana
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado