Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves

Author(s)

Santana, Andre M. ^[1] ; Silva, Daniela G. ^[1] ; Thomas, Funmilola C. ^[2] ; Bernardes, Priscila A. ^[1] ; Pizauro, Lucas J. L. ^[1] ; Santana, Clarissa H. ^[1] ; Burchmore, Richard J. S. ^[3] ; Eckersall, Peter D. ^[4] ; Fagliari, Jose J. ^[1]

Affiliation

^[1] Sao Paulo State Univ FCAV UNESP, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabtil, SP - Brazil

^[2] Fed Univ Agr, Coll Vet Med, Dept Vet Physiol & Pharmacol, Abeokuta - Nigeria

^[3] Univ Glasgow, Glasgow Poly Facil, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow, Lanark - Scotland

^[4] Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Coll Med Vet & Life Sci, Glasgow, Lanark - Scotland

• Document type: Journal article
• Source: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 203, p. 30-39, SEP 2018.
• Web of Science Citations: 0

Abstract

The study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 10(8) CFU of S. Dublin (INF). Clinical evaluation was performed, rectal swabs to detect S. Dublin strains were collected and venous blood was sampled before and throughout seven days after inoculation. The APP fractions beta-haptoglobin, alpha-haptoglobin, ceruloplasmin and transferrin were analyzed by 1-D and 2-D electrophoresis. Proteins were identified using LC/ESI-MS/MS and NCBI database. Plasma fibrinogen, serum iron and serum haptoglobin concentrations were measured. The inoculation of 10(8) CFU of S. Dublin was effective in inducing clinical signs of Salmonellosis, such as hyperthermia and diarrhea. 1-DE showed that beta and alpha-haptoglobin increased 204% (p = 0.008) and 184% (p = 0.022) 48 h after inoculation (HAI), respectively, with highest concentrations 120 HAI (498% increased, p = 0.012; 431% increased, p = 0.011) and 168 HAI (492% increased, p = 0.019; 523% increased, p = 0.028). 2-DE showed that the expression of two spots, identified as beta-haptoglobin, were increased 693% (p = 0.0006) and 580% (p = 0.0003) 168 HAI, respectively, while one spot, identified as alpha-haptoglobin, increased 714% (p = 0.040). Haptoglobin concentrations increased 1339% (p < 0.0001) 168 HAI. 1-DE showed that ceruloplasmin increased 42% (p = 0.034) 48 HAI, with highest concentration 120 HAI (133% increased, p = 0.022). 2-DE showed that the expression of two spots, identified as ceruloplasmin, were increased 218% (p = 0.0153) and 85% (p = 0.0143) 168 HAI, respectively. Fibrinogen increased 78% (p = 0.012) 96 HAI, with highest concentration 120 HAI (increased 114%, p = 0.002). Iron decreased 33% 24 HAI (p = 0.015) and 37% 72 HAI (p = 0.029), and began to be restored 96 HAI. 1-DE showed that transferrin decreased 23% 120 HAI (p = 0.047), and that values were restored 168 HAI. 2-DE showed that expression patterns of transferrin comparing Oh and 168 HAI were similar, evidencing that values were restored 168 HAI. In conclusion, the inoculation of 10(8) CFU was effective in inducing hyperthermia and diahrrea. beta and alpha-haptoglobin, ceruloplasmin and fibrinogen worked as positive APP during the APR to S. Dublin infection and are potential biomarker candidates. Concentrations of iron and transferrin decreased during the infection, highlighting the fact that mechanisms for restricting iron availability are part of the APR triggered against S. Dublin infection in buffalo calves. (AU)

• FAPESP's process: 09/05410-7 - Clinical, hemogram and serum protein concentration avaliation, with emphasis in acute phase proteins and immunoglobulins, of buffalo calves infected experimentaly by Salmonella enterica subspecies enterica sorovar Dublin
• Grantee: Priscila Arrigucci Bernardes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 09/12350-0 - Clinical, laboratorial and therapeutic study of experimental infection of bufallo calves by Salmonella dublin
• Grantee: José Jurandir Fagliari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/26498-5 - Blood serum and milk whey acute phase proteins and proteomic studies in buffalo and bovine: mastitis and salmonellosis
• Grantee: André Marcos Santana
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 09/05211-4 - Clinical, serum biochemistry and blood gas analysis of buffalo calves infected experimentaly by Salmonella enterica subspecies enterica sorovar Dublin
• Grantee: Lucas José Luduverio Pizauro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/10094-0 - Biochemical profile of milk secretion, including acute phase proteins and enzymes NAGase and LDH, as a method of diagnosis and prognosis of subclinical and clinical mastitis in female cattle and buffalo
• Grantee: André Marcos Santana
• Support Opportunities: Scholarships in Brazil - Post-Doctoral