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Serum biochemical profile, including proteinogram with emphasis on acute phase proteins and immunoglobulins in neonates buffalo calves

Grant number: 14/09246-5
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:José Jurandir Fagliari
Grantee:Damazio Campos de Souza
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


This study aims to evaluate the serum biochemical profile, with an emphasis on acute phase proteins and immunoglobulins of healthy Murrah buffalo calves neonates. Blood serum samples of 50 animals Murrah derived from a farm producing milk in the municipality of Sarapuí-SP will be examined. The buffalo calves will be divided into two experimental groups of 25 animals each, according to the number of calvings from their mothers: G1 (calves of primiparous buffaloes) and G2 (multiparous buffalo calves). Sampling of venous blood samples will be held at the following times: at birth (T0), at 24 (M1), 48h (M2) and 72h after birth (M3) and 7 (M4), 14 (M5), 21 (M6) and 30 days of age (M7) for determination of serum activities of Gamaglutamiltranferase Enzymes (GGT), Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST) and Creatine Kinase (CK) and serum concentrations of total protein, albumin, iron, total calcium, ionized calcium, phosphorus, sodium and potassium. Serum concentrations of acute phase proteins and immunoglobulins will be determined by electrophoresis in polyacrylamide gel containing sodium dodecyl sulfate (SDS-PAGE). The values obtained will be analyzed by Tukey test after checking the homogeneity of the samples. The significance will be verified at 5% probability. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUZA, D. C.; SILVA, D. G.; ROCHA, T. G.; MONTEIRO, B. M.; PEREIRA, G. T.; FIORI, L. C.; VIANA, R. B.; FAGLIARI, J. J. Serum biochemical profile of neonatal buffalo calves. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 71, n. 1, p. 187-196, JAN-FEB 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Damazio Campos de. Perfil bioquímico sérico de bezerros bubalinos no período neonatal. 2016. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

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