Clinical impact of Candida spp. biofilm production in a cohort of patients with candidemia

Autor(es)

Monfredini, Priscilla Morais ^[1] ; Remondi Souza, Ana Carolina ^[1] ; Cavalheiro, Renan Pelluzzi ^[2] ; Siqueira, Ricardo Andreotti ^[1] ; Colombo, Arnaldo Lopes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Infectol, Lab Especial Micol, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Biol Mol, Dept Bioquim, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 56, n. 7, p. 803-808, OCT 2018.
• Citações Web of Science: 3

Resumo

Persistent candidemia refers to the continued isolation of the same Candida species in the blood of a candidemic patient despite appropriate therapy. Despite the clinical importance of persistent candidemia, studies have superficially addressed the biological conditions behind this phenomenon. The aim of this studywas to evaluate the correlation between the biofilm-forming ability by Candida bloodstream isolates and the persistence of infection. A total of 55 isolates of Candida were tested and characterized in two groups: (i) group I, which included seven patients with persistent candidemia, and (ii) group II, which included 18 patients with nonpersistent candidemia. Microorganisms were identified at the species level by sequencing the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). Biofilm quantification was evaluated by the crystal violet staining method and confocal scanning laser microscopy (CSLM). Molecular tests confirmed the identification of Candida albicans (92% group I and 94% group II) and Candida dubliniensis isolates (8% group I and 6% group II). All 55 isolates were able to form biofilms, but a higher biofilm mass was produced by C. albicans/C. dubliniensis strains cultured from the persistent group (P<.05). Our data suggest that Candida sp. biofilm production should be considered a relevant biologic variable in explaining patients who fail to clear a bloodstream infection despite adequate antifungal treatment with triazoles. (AU)

• Processo FAPESP: 12/04767-1 - Complexo Candida parapsilosis: peculiaridades epidemiológicas, virulência e mecanismos de resistência aos antifúngicos
• Beneficiário: Arnaldo Lopes Colombo
• Modalidade de apoio: Auxílio à Pesquisa - Regular