Absence of seminal plasma from sperm-rich fraction decreases boar sperm quality characteristics during the course of liquid storage

Autor(es)

Leal, D. F. ^[1] ; Torres, M. A. ^[1] ; Ravagnani, G. M. ^[1] ; Martins, S. M. M. K. ^[1] ; Meirelles, F. V. ^[2] ; de Andrade, A. F. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Swine Res Ctr, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction Science; v. 198, p. 20-26, NOV 2018.
• Citações Web of Science: 1

Resumo

Seminal plasma (SP), the fluid that surrounds the sperm cells, is known to exert substantial influence on sperm physiology. The SP has a pivotal role in sperm function in vivo, and due to its components, it functions in an ambiguous manner in vitro, simultaneously possessing deleterious and beneficial effects. This experiment aimed to describe the differences between the presence or absence of SP from the sperm-rich fraction on some spermatozoa characteristics (kinetics, plasma and acrosome membrane integrity, lipid peroxidation and capacitation-like changes). Furthermore, this experiment focused on distinguishing the effects of SP on the variables evaluated from the effects of centrifugation during SP removal. Total and progressive sperm motility, as well as integrity of plasma and acrosome membranes, were less (P < 0.05) in the absence of SP. Membrane lipid peroxidation (P < 0.05) and sperm membrane stability (P < 0.05) did not differ among treatments. The SP from the sperm-rich fraction is important for the maintenance of adequate structural and functional characteristics of extended liquid boar semen and should be present in seminal doses throughout storage. Furthermore, the detrimental effect on the variables evaluated was caused solely by the absence of SP and not by the process of removal through centrifugation at 500 x g for 10 min. (AU)

• Processo FAPESP: 14/20768-3 - Influência do plasma seminal oriundo da fração rica do ejaculado sobre as características estruturais e na cinética do espermatozóide suíno conservado sob refrigeração a 17°C por 72 horas
• Beneficiário: Diego Feitosa Leal
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/14258-5 - Influência do plasma seminal oriundo da fração rica do ejaculado sobre as características estruturais e cinéticas, bem como na capacitação e na fertilidade do espermatozoide suíno conservado sob refrigeração à 17°C por 72 horas
• Beneficiário: André Furugen Cesar de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular