Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh platelet-rich plasma

Autor(es)

da Silva, Leticia Queiroz ^[1] ; de Lima Montalvao, Silmara Aparecida ^[1] ; Justo-Junior, Amauri da Silva ^[2] ; Rosenberis Cunha Junior, Jose Luiz ^[1] ; Huber, Stephany Cares ^[1] ; Oliveira, Carolina Caliari ^[3] ; Annichino-Bizzacchi, Joyce Maria ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Hemoctr, Haemostasis Lab, BR-13083970 Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Dept Clin Pathol, Fac Med Sci, BR-13083970 Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Fac Med Sci, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REGENERATIVE MEDICINE; v. 13, n. 7, p. 775-784, OCT 2018.
• Citações Web of Science: 1

Resumo

Aims: To compare levels and activity of the growth factors between fresh and lyophilized platelet-rich plasma (PRP). Methods: Analysis of platelet concentration using fibroblast and human umbilical vein endothelial cell cultures were compared between fresh and lyophilized PRP obtained from peripheral blood. Results: After lyophilization, 54% of platelets were intact whereas the fresh showed no aggregation with agonists (levels under 20%). The concentration of growth factors (VEGF, EGF, TGF- and PDGF) in both products were similar. Fresh and lyophilized PRPs induced proliferation in the fibroblasts at 24h (0.303 vs 0.300, respectively). Conclusion: Lyophilized PRP appears to be an alternative to fresh PRP and the results evidenced the role of growth factors as a key element in the activity of this product. (AU)

• Processo FAPESP: 15/05544-4 - Caracterização in vitro e funcional do plasma rico em plaqueta (PRP) autólogo, como produto terapêutico em diferentes condições de processamento
• Beneficiário: Letícia Queiroz da Silva Justo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica