Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh platelet-rich plasma

Author(s)

da Silva, Leticia Queiroz ^[1] ; de Lima Montalvao, Silmara Aparecida ^[1] ; Justo-Junior, Amauri da Silva ^[2] ; Rosenberis Cunha Junior, Jose Luiz ^[1] ; Huber, Stephany Cares ^[1] ; Oliveira, Carolina Caliari ^[3] ; Annichino-Bizzacchi, Joyce Maria ^[1]

Affiliation

^[1] State Univ Campinas UNICAMP, Hemoctr, Haemostasis Lab, BR-13083970 Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Dept Clin Pathol, Fac Med Sci, BR-13083970 Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Fac Med Sci, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: REGENERATIVE MEDICINE; v. 13, n. 7, p. 775-784, OCT 2018.
• Web of Science Citations: 1

Abstract

Aims: To compare levels and activity of the growth factors between fresh and lyophilized platelet-rich plasma (PRP). Methods: Analysis of platelet concentration using fibroblast and human umbilical vein endothelial cell cultures were compared between fresh and lyophilized PRP obtained from peripheral blood. Results: After lyophilization, 54% of platelets were intact whereas the fresh showed no aggregation with agonists (levels under 20%). The concentration of growth factors (VEGF, EGF, TGF- and PDGF) in both products were similar. Fresh and lyophilized PRPs induced proliferation in the fibroblasts at 24h (0.303 vs 0.300, respectively). Conclusion: Lyophilized PRP appears to be an alternative to fresh PRP and the results evidenced the role of growth factors as a key element in the activity of this product. (AU)

• FAPESP's process: 15/05544-4 - Characterization in vitro and functional of autologous platelet-rich plasma (PRP) as a therapeutic product in different processing conditions
• Grantee: Letícia Queiroz da Silva Justo
• Support Opportunities: Scholarships in Brazil - Scientific Initiation