Cryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viability

Autor(es)

Ravagnani, Gisele M. ^[1] ; Torres, Mariana A. ^[1] ; Leal, Diego F. ^[1] ; Martins, Simone M. M. K. ^[1] ; Papa, Frederico O. ^[2] ; Dell'Aqua Junior, Jose A. ^[2] ; Alvarenga, Marco A. ^[2] ; Andrade, Andre F. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Nucl Pesquisa Suinos, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, UNESP, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Pesquisa Veterinária Brasileira; v. 39, n. 9, p. 1726-1730, SEP 2018.
• Citações Web of Science: 1

Resumo

To date, no studies have been performed evaluating the effect of boar spermatozoa concentration in 0.5mL freezing straws, leading us to examine this question. Each sperm-rich fraction of the ejaculate (n=25) was diluted at five different sperm concentrations (100, 200, 300, 600 and 800 x 10(6) spermatozoa/mL), packaged in 0.5mL straws, and subsequently frozen. After thawing, the sperm from all of treatment groups were analyzed to determine motility characteristics using a sperm class analyzer (SCA-CASA), and their plasma and acrosomal membrane integrity, mitochondria! membrane potential, sperm membrane lipid peroxidation and fluidity were analyzed by flow cytometry. An increase in spermatozoa concentration above 300x10(6) spermatozoa/mL in a 0.5mL straw impaired (p<0.05) the total and progressive motility, curvilinear velocity, straight-line velocity, linearity and beat cross frequency. However, the plasma and acrosomal membrane integrity, mitochondria] membrane potential, membrane lipid peroxidation and fluidity were not influenced (p>0.05) by high spermatozoa concentrations at freezing. Therefore, to increase spermatozoa survival and total and progressive motility after thawing, boar spermatozoa should be frozen at concentrations up to 300x10(6) spermatozoa/mL. (AU)

• Processo FAPESP: 11/23484-8 - Efeito do plasma seminal na inibição/reversão das mudanças semelhantes à capacitação e na fertilidade dos espermatozóides criopreservados de suínos
• Beneficiário: André Furugen Cesar de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular