Characterization of molecular biomarkers of mercury exposure to muscle tissue of Plagioscion squamosissimus and Colossoma macropomum from the Amazon region

Autor(es)

Bittarello, Alis Correia ^[1] ; Souza Vieira, Jose Cavalcante ^[2] ; Braga, Camila Pereira ^[3] ; de Paula Araujo, Wellington Luiz ^[1] ; Bataglioli, Izabela da Cunha ^[1] ; da Silva, Janaina Macedo ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[4] ; Fleuri, Luciana Francisco ^{[2, 1]} ; Padilha, Pedro de Magalhaes ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] UNESP, FMVZ, Grad Program Anim Sci, Botucatu, SP - Brazil

^[2] UNESP, Inst Biosci, Botucatu, SP - Brazil

^[3] Univ Nebraska, UNL, Lincoln, NE - USA

^[4] Univ Sao Paulo, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 276, p. 247-254, MAR 15 2019.
• Citações Web of Science: 3

Resumo

Mercury has the ability to bind to a variety of biomolecules, which can compromise its structure and functionality and thus promote its toxic effects. The aim of this study is to identify possible mercury biomarkers in muscle samples of Plagioscion squamosissimus (carnivorous fish) and Colossoma macropomum (omnivorous fish), from the Amazon region. The muscle proteome of fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and the total mercury concentrations in protein spots were determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein spots containing mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The mercury concentrations in the protein spots were in the range of 1.10 +/- 0.02-23.90 +/- 0.33 mu g g(-1). The proteins phosphoglycerate mutase 2 (P. squamosissimus), hemoglobin beta and cytochrome P450scc (C. macropomum), identified by ESI-MS/MS and showing the highest values of mercury concentration, may be considered possible mercury biomarkers. (AU)

• Processo FAPESP: 16/19404-2 - Caracterização de biomarcadores de toxicidade de mercúrio em peixes da Bacia do Rio Madeira-Rondônia utilizando-se estratégias metaloproteômicas
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02668-1 - Utilização de estratégias metaloproteômicas para avaliação de metalotioneínas em amostras de tecido hepático de peixes coletados no reservatório do Ahe Jirau - Bacia do Rio Madeira, RO, Brasil
• Beneficiário: José Cavalcante Souza Vieira
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular