Characterization of molecular biomarkers of mercury exposure to muscle tissue of Plagioscion squamosissimus and Colossoma macropomum from the Amazon region

Author(s)

Bittarello, Alis Correia ^[1] ; Souza Vieira, Jose Cavalcante ^[2] ; Braga, Camila Pereira ^[3] ; de Paula Araujo, Wellington Luiz ^[1] ; Bataglioli, Izabela da Cunha ^[1] ; da Silva, Janaina Macedo ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[4] ; Fleuri, Luciana Francisco ^{[2, 1]} ; Padilha, Pedro de Magalhaes ^{[2, 1]}

Affiliation

^[1] UNESP, FMVZ, Grad Program Anim Sci, Botucatu, SP - Brazil

^[2] UNESP, Inst Biosci, Botucatu, SP - Brazil

^[3] Univ Nebraska, UNL, Lincoln, NE - USA

^[4] Univ Sao Paulo, Bauru, SP - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 276, p. 247-254, MAR 15 2019.
• Web of Science Citations: 3

Abstract

Mercury has the ability to bind to a variety of biomolecules, which can compromise its structure and functionality and thus promote its toxic effects. The aim of this study is to identify possible mercury biomarkers in muscle samples of Plagioscion squamosissimus (carnivorous fish) and Colossoma macropomum (omnivorous fish), from the Amazon region. The muscle proteome of fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and the total mercury concentrations in protein spots were determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein spots containing mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The mercury concentrations in the protein spots were in the range of 1.10 +/- 0.02-23.90 +/- 0.33 mu g g(-1). The proteins phosphoglycerate mutase 2 (P. squamosissimus), hemoglobin beta and cytochrome P450scc (C. macropomum), identified by ESI-MS/MS and showing the highest values of mercury concentration, may be considered possible mercury biomarkers. (AU)

• FAPESP's process: 16/19404-2 - Mercury toxicity biomarker characterization in fish from Basin of the Madeira River-Rondônia using metalloproteomic strategies
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02668-1 - Utilization of metalloproteomic strategies for metallothionein evaluation in liver tissue samples of fishes collected in the reservoir of Ahe Jirau - Madeira River Basin, RO, Brazil
• Grantee: José Cavalcante Souza Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/21297-1 - Metallothioneins in fish from Amazon Region: metallomic study associated to mercury
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants