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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of molecular biomarkers of mercury exposure to muscle tissue of Plagioscion squamosissimus and Colossoma macropomum from the Amazon region

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Bittarello, Alis Correia [1] ; Souza Vieira, Jose Cavalcante [2] ; Braga, Camila Pereira [3] ; de Paula Araujo, Wellington Luiz [1] ; Bataglioli, Izabela da Cunha [1] ; da Silva, Janaina Macedo [2] ; Rabelo Buzalaf, Marilia Afonso [4] ; Fleuri, Luciana Francisco [2, 1] ; Padilha, Pedro de Magalhaes [2, 1]
Total Authors: 9
[1] UNESP, FMVZ, Grad Program Anim Sci, Botucatu, SP - Brazil
[2] UNESP, Inst Biosci, Botucatu, SP - Brazil
[3] Univ Nebraska, UNL, Lincoln, NE - USA
[4] Univ Sao Paulo, Bauru, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Food Chemistry; v. 276, p. 247-254, MAR 15 2019.
Web of Science Citations: 3

Mercury has the ability to bind to a variety of biomolecules, which can compromise its structure and functionality and thus promote its toxic effects. The aim of this study is to identify possible mercury biomarkers in muscle samples of Plagioscion squamosissimus (carnivorous fish) and Colossoma macropomum (omnivorous fish), from the Amazon region. The muscle proteome of fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and the total mercury concentrations in protein spots were determined by graphite furnace atomic absorption spectrometry (GFAAS). The protein spots containing mercury were characterized by electrospray ionization tandem mass spectrometry (ESI-MS/MS). The mercury concentrations in the protein spots were in the range of 1.10 +/- 0.02-23.90 +/- 0.33 mu g g(-1). The proteins phosphoglycerate mutase 2 (P. squamosissimus), hemoglobin beta and cytochrome P450scc (C. macropomum), identified by ESI-MS/MS and showing the highest values of mercury concentration, may be considered possible mercury biomarkers. (AU)

FAPESP's process: 13/21297-1 - Metallothioneins in fish from Amazon Region: metallomic study associated to mercury
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants
FAPESP's process: 16/19404-2 - Mercury toxicity biomarker characterization in fish from Basin of the Madeira River-Rondônia using metalloproteomic strategies
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants
FAPESP's process: 14/02668-1 - Utilization of metalloproteomic strategies for metallothionein evaluation in liver tissue samples of fishes collected in the reservoir of Ahe Jirau - Madeira River Basin, RO, Brazil
Grantee:José Cavalcante Souza Vieira
Support type: Scholarships in Brazil - Doctorate