Multi-walled carbon nanotubes used as support for lipase from Burkholderia cepacia: Morphological and physicochemical properties

Autor(es)

Padilha, G. S. ^[1] ; Campos, V. A. B. ^[2] ; Costa, M. C. ^[3] ; Franco, T. T. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Res Grp Mfg Adv Mat, Sch Appl Sci FCA, 1300 Pedro Zaccaria St, BR-13484350 Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Sch Chem Engn, Dept Proc Engn DEPRO, Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Sch Chem Engn, Dept Proc & Prod Dev DDPP, Albert Einstein 500, BR-13083852 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY; v. 134, n. 2, p. 1021-1029, NOV 2018.
• Citações Web of Science: 2

Resumo

Commercial lipase from Burkholderia cepacia is immobilized on functionalized multi-walled carbon nanotubes (MWNT-COOH and MWNT-OH) provided by a physical adsorption. The immobilization processes for the carbon nanotubes are defined using immobilization time (0-30min) and distinct adsorbent:adsorbate ratios (1:4, 1:7, and 1:10) with lipase loading of 100, 175, and 250mg, respectively. The characterization of the immobilized preparations, the free lipase, and the pure nanotubes (MWNT-COOH and MWNT-OH) indicate that the lipase adsorption is increased. Thermogravimetric analysis, differential scanning calorimetry, and scanning electron microscopy are used. The specific surface area, pore volumes, and average pore diameters are determined by nitrogen adsorption-desorption isotherms. For the pure lipase, in the range between 40 and 300 degrees C, the micrograph is acquired. Experimental results clearly show an effective lipase adsorption in a lower period of time (5min) in MWNT-COOH and MWNT-OH as well as a decrease in the surface area (98.30-45.9(86)+/- 2.5 and 97.61-37.71 +/- 3.3(7)m(2)g(-1)) and the pore volume (0.48-0.25 +/- 0.01 and 0.39-0.24 +/- 0.05cm(3)g(-1)), indicating that functionalized multi-walled carbon nanotubes can be successfully used as enzyme support. (AU)

• Processo FAPESP: 12/05027-1 - Equilíbrio sólido-líquido de compostos graxos e ponto de fulgor de biodiesel
• Beneficiário: Mariana Conceição da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores