Novel Insights Into the Early Stages of Ratoon Stunting Disease of Sugarcane Inferred from Transcript and Protein Analysis

Autor(es):Mostrar menos -

Cia, Mariana Cicarelli ^[1] ; de Carvalho, Giselle ^[1] ; Azevedo, Ricardo Antunes ^[1] ; Monteiro-Vitorello, Claudia Barros ^[1] ; Souza, Glaucia Mendes ^[2] ; Nishiyama-Junior, Milton Yutaka ^[3] ; Lembke, Carolina Gimiliani ^[2] ; da Cunha Antunes de Faria, Raphael Severo ^[1] ; Rodrigues Marques, Joao Paulo ^[1] ; Melotto, Maeli ^[4] ; Aranha Camargo, Luis Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Ave Prof Lineu Prestes 748, BR-05508900 Sao Paulo, SP - Brazil

^[3] Inst Butantan, Lab Especial Toxinol Aplicada, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[4] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 - USA

• Tipo de documento: Artigo Científico
• Fonte: PHYTOPATHOLOGY; v. 108, n. 12, p. 1455-1466, DEC 2018.
• Citações Web of Science: 0

Resumo

Despite of the importance of ratoon stunting disease, little is known on the responses of sugarcane to its causal agent, the vascular bacterial endophyte Leifsonia xyli subsp. xyli. The transcriptome and proteome of young plants of a susceptible cultivar with no symptoms of stunting but with relative low and high bacterial titers were compared at 30 and 60 days after inoculation. Increased bacterial titers were associated with alterations in the expression of 267 cDNAs and in the abundance of 150 proteins involved in plant growth, hormone metabolism, signal transduction and defense responses. Some alterations are predicted to benefit the pathogen, such as the up-regulation of genes involved in the synthesis of methionine. Also, genes and proteins of the cell division cycle were all down-regulated in plants with higher titers at both times. It is hypothesized that the negative effects on cell division related to increased bacterial titers is cumulative over time and its modulation by other host and environmental factors results in the stunting symptom. (AU)

• Processo FAPESP: 12/25315-1 - Histopatologia da interação - Sporisorium scitamineum vs. cana-de-açúcar
• Beneficiário: João Paulo Rodrigues Marques
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/56260-2 - Functional'omics of the ratoon stunting disease of sugarcane
• Beneficiário: Luis Eduardo Aranha Camargo
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 15/18681-0 - Análises funcionais no patossistema cana-de-açúcar - Leifsonia xyli subsp. xyli: alterações metabólicas e celulares no hospedeiro e respostas a estresse oxidativo e osmótico no patógeno
• Beneficiário: Luis Eduardo Aranha Camargo
• Linha de fomento: Auxílio à Pesquisa - Regular