| Texto completo | |
| Autor(es): |
Sgro, German G.
[1, 2, 3]
;
Costa, Tiago R. D.
[2, 3, 4]
;
Cenens, William
[1]
;
Souza, Diorge P.
[1, 5]
;
Cassago, Alexandre
[6]
;
de Oliveira, Luciana Coutinho
[1, 7]
;
Salinas, Roberto K.
[1]
;
Portugal, V, Rodrigo
;
Farah, Chuck S.
[1]
;
Waksman, Gabriel
[2, 3, 8]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[2] UCL, Inst Struct & Mol Biol, London - England
[3] Birkbeck Coll, Dept Biol Sci, London - England
[4] Imperial Coll London, Dept Life Sci, MRC Ctr Mol Bacteriol & Infect, London - England
[5] UCL, MRC Lab Mol Cell Biol, London - England
[6] V, CNPEM, Lab Nacl Nanotecnol LNNano, Campinas, SP - Brazil
[7] Univ Quebec, Dept Med Chem, INRS Inst Armand Frappier, Laval, PQ - Canada
[8] UCL, Div Biosci, Res Dept Struct & Mol Biol, Birkbeck Coll, London - England
Número total de Afiliações: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NATURE MICROBIOLOGY; v. 3, n. 12, p. 1429-1440, DEC 2018. |
| Citações Web of Science: | 11 |
| Resumo | |
Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 angstrom-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein-protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution. (AU) | |
| Processo FAPESP: | 17/17303-7 - Estrutura e função de sistemas de secreção bacterianas |
| Beneficiário: | Shaker Chuck Farah |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 11/07777-5 - Sinalização por c-di-GMP e o sistema de secreção de macromoléculas do tipo IV em Xanthomonas citri |
| Beneficiário: | Shaker Chuck Farah |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |