Forced Swim Reliability for Exercise Testing in Rats by a Tethered Swimming Apparatus

Autor(es)

dos Reis, Ivan G. M. ^[1] ; Martins, Luiz E. B. ^[2] ; de Araujo, Gustavo G. ^[1] ; Gobatto, Claudio A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Appl Sport Physiol, Limeira - Brazil

^[2] Univ Estadual Campinas, Sch Phys Educ, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PHYSIOLOGY; v. 9, DEC 19 2018.
• Citações Web of Science: 1

Resumo

To assess the physical capacity of rats in forced swim tests, the animal should perform a continuous activity (CON) at the surface to avoid apnea. Bobbing movement (BOB), vigorous paddling known as climbing (CLI), and diving activity (DIV) are inadequate swimming patterns known to increase the exercise intensity variability, impairing the test reliability. Thus, the exercise work accomplished and related physiological variables, such as the blood lactate concentration, may be unreproducible in forced swim. This study aimed to verify the exercise work reproducibility in rats with a 30-min test-retest at maximal lactate steady state (MLSS) intensity using a tethered-swimming apparatus that analyzes swimming patterns by the direct measurement of swimming force. Additionally, it was determined the swimming force and duration of CON, BOB, CLI, and DIV at physiologically different exercise-intensities. The swimming force at MLSS (n = 64) was 38 +/- 7 gf.Kg(-1), while the blood lactate concentration was 4.2 +/- 1.6 mmol.L-1. In the test-retest (N = 23), swimming force (36.6 +/- 7 gf.Kg(-1) vs. 36.4 +/- 7 gf.Kg(-1)) and blood lactate concentration (4.7 +/- 1.7 mmol.L-1 vs. 4.2 +/- 1.7 mmol.l(-1)) were similar, but only the swimming force was highly correlated (0.90 and 0.31). Although it was not statistically different, the swimming force for CON tends to be slightly lower than CLI and slightly higher than BOB independently of exercise-intensity. The CON pattern predominates (similar to 52.8 +/- 18%) at intensities below and of MLSS but BOB was the swimming pattern more often observed above MLSS-intensity (52.6 +/- 18%). The present study used a tethered swimming apparatus to investigate the reliability of forced swim tests for exercise testing in rats and better understand the swimming patterns when determining the MLSS, but the results can be extended to any study that rely on forced swim for exercise testing and training. The result suggests that, at least at intensities of physiological stability, the exercise work accomplished by rats is reproducible in forced swim, but the blood lactate concentration seems to be affected by other factors, such as the apnea and stress caused by the possibility of drowning, besides the exercise-intensity. (AU)

• Processo FAPESP: 06/61189-0 - Padronizacao de um metodo para determinacao da densidade corporal e sobrecarga no modelo de natacao em ratos wistar.
• Beneficiário: Ivan Gustavo Masselli dos Reis
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/08284-2 - EFEITO AGUDO DO EXERCÍCIO EM INTENSIDADE EQUIVALENTE E ACIMA DA MÁXIMA FASE ESTÁVEL DE LACTATO NAS EXPRESSÕES PROTEICAS E mRNAs DE HIF-1±, MCTs 1 E 4 E PGC-1±, EM TECIDO CARDÍACO, HEPÁTICO E MUSCULAR ESQUELÉTICO DE RATOS NADADORES
• Beneficiário: Ivan Gustavo Masselli dos Reis
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/03445-5 - Análise de parâmetros de força e padrão de nado de ratos controles e obesos, a partir de um sistema de aquisição de sinais em natação atada. influência da intensidade do exercício e da densidade corporal
• Beneficiário: Ivan Gustavo Masselli dos Reis
• Modalidade de apoio: Bolsas no Brasil - Mestrado