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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Encapsulation of collagenase within biomimetically mineralized metal-organic frameworks: designing biocomposites to prevent collagen degradation

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Autor(es):
Bim Junior, Odair [1] ; Bedran-Russo, Ana [2] ; Flor, Jader B. S. [3] ; Borges, Ana F. S. [4] ; Ximenes, Valdecir F. [5] ; Frem, Regina C. G. [3] ; Lisboa-Filho, Paulo N. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP Sao Paulo State Univ, Sch Sci, Dept Phys, Bauru - Brazil
[2] UIC, Coll Dent, Dept Restorat Dent, Chicago, IL - USA
[3] UNESP Sao Paulo State Univ, Inst Chem, Dept Inorgan Chem, Araraquara - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru - Brazil
[5] UNESP Sao Paulo State Univ, Sch Sci, Dept Chem, Bauru - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: NEW JOURNAL OF CHEMISTRY; v. 43, n. 2, p. 1017-1024, JAN 14 2019.
Citações Web of Science: 0
Resumo

A growing class of multifunctional porous materials termed metal-organic frameworks (MOFs) has attracted attention for immobilization of biomolecules via self-assembly of inorganic and organic building blocks. Herein, we report the rapid formation of MOF shells surrounding collagen-degrading enzymes by using a biomimetic mineralization approach. Bacterial collagenase, which is functionally related to endogenous proteases in the human body, was used as a nucleating agent to induce the mineralization of zeolitic imidazolate framework-8 (ZIF-8) as a crystalline shell. This zinc-based MOF material was selected due to its remarkable stability under physiological conditions and good biocompatibility. The straightforward, water-based synthesis yielded microporous collagenase-embedded ZIF-8 particles. Once immobilized inside the biomimetically mineralized MOF, the protease presented limited catalytic activity, being ineffective in the proteolysis of a collagen-like peptide. In conclusion, biomimetic mineralization of ZIF-8 using collagenase not only provided immobilization of the enzyme, but also enabled the control of its activity. This synthetic approach provides a potentially useful concept in the prevention of proteolytic activity involved in the degradation of collagen matrices in living organisms. (AU)

Processo FAPESP: 18/02186-8 - Uso de MOFs (metal-organic frameworks) automontáveis na prevenção da degradação da matriz dentinária: cápsulas cristalinas para proteases e revestimentos protetores para fibrilas de colágeno
Beneficiário:Odair Bim Júnior
Linha de fomento: Bolsas no Brasil - Pós-Doutorado