| Full text | |
| Author(s): |
Bim Junior, Odair
[1]
;
Bedran-Russo, Ana
[2]
;
Flor, Jader B. S.
[3]
;
Borges, Ana F. S.
[4]
;
Ximenes, Valdecir F.
[5]
;
Frem, Regina C. G.
[3]
;
Lisboa-Filho, Paulo N.
[1]
Total Authors: 7
|
| Affiliation: | [1] UNESP Sao Paulo State Univ, Sch Sci, Dept Phys, Bauru - Brazil
[2] UIC, Coll Dent, Dept Restorat Dent, Chicago, IL - USA
[3] UNESP Sao Paulo State Univ, Inst Chem, Dept Inorgan Chem, Araraquara - Brazil
[4] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru - Brazil
[5] UNESP Sao Paulo State Univ, Sch Sci, Dept Chem, Bauru - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | NEW JOURNAL OF CHEMISTRY; v. 43, n. 2, p. 1017-1024, JAN 14 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
A growing class of multifunctional porous materials termed metal-organic frameworks (MOFs) has attracted attention for immobilization of biomolecules via self-assembly of inorganic and organic building blocks. Herein, we report the rapid formation of MOF shells surrounding collagen-degrading enzymes by using a biomimetic mineralization approach. Bacterial collagenase, which is functionally related to endogenous proteases in the human body, was used as a nucleating agent to induce the mineralization of zeolitic imidazolate framework-8 (ZIF-8) as a crystalline shell. This zinc-based MOF material was selected due to its remarkable stability under physiological conditions and good biocompatibility. The straightforward, water-based synthesis yielded microporous collagenase-embedded ZIF-8 particles. Once immobilized inside the biomimetically mineralized MOF, the protease presented limited catalytic activity, being ineffective in the proteolysis of a collagen-like peptide. In conclusion, biomimetic mineralization of ZIF-8 using collagenase not only provided immobilization of the enzyme, but also enabled the control of its activity. This synthetic approach provides a potentially useful concept in the prevention of proteolytic activity involved in the degradation of collagen matrices in living organisms. (AU) | |
| FAPESP's process: | 18/02186-8 - Use of self-assembled metal-organic frameworks (MOFs) in preventing degradation of dentin matrix: crystalline shells for proteases and protective coatings for collagen fibrils |
| Grantee: | Odair Bim Júnior |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |