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Use of self-assembled metal-organic frameworks (MOFs) in preventing degradation of dentin matrix: crystalline shells for proteases and protective coatings for collagen fibrils

Grant number: 18/02186-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): April 01, 2018
Effective date (End): March 31, 2020
Field of knowledge:Engineering - Materials and Metallurgical Engineering
Principal Investigator:Paulo Noronha Lisboa Filho
Grantee:Odair Bim Júnior
Home Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
Associated research grant:13/07296-2 - CDMF - Center for the Development of Functional Materials, AP.CEPID


Biodegradation of the dentin collagen matrix by intrinsic enzymes, such as matrix metalloproteinases (MMPs), is one of the factors limiting the durability of dental restorations made with resin-based materials. Controlling the enzyme bioactivity and protecting the susceptible collagen are suitable strategies for preventing the collagenolytic activity in the dentin matrix. This research intends to investigate the unprecedented use of nanoporous, crystalline materials termed metal-organic frameworks (MOFs) in preventing the degradation of dentin collagen. Recently, it has been demonstrated that biomolecules like enzymes and proteins are able to induce the mineralization of protective MOF coatings around them via biomimetic processes. We posit that MOFs can be synthetized around collagenases (as crystalline shells) and collagen fibrils (as protective coatings). The main objective is to design biocomposites that present controllable bioactivity (as for collagenase encapsulated by MOF) and resistance to proteolysis (as for collagen coated with MOF). The synthesized biocomposites (e.g., MOF/collagenase and MOF/collagen biocomposites) are expected to be stable under physiological conditions and unable to develop collagenolytic activity. Zeolitic imidazolate framework-8 (ZIF-8) is selected as a candidate MOF material in this study due to its chemical stability, good biocompatibility and insignificant cytotoxicity. MOF/collagenase and MOF/collagen biocomposites will be characterized with respect to their chemical composition, structure, morphology, bioactivity and biostability. We hope to identify an innovative proof-of-concept approach regarding the application of biomimetically mineralized protective MOFs in the bioengineering of biomacromolecules related functionally to the dentin matrix.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BIM JUNIOR, ODAIR; BEDRAN-RUSSO, ANA; FLOR, JADER B. S.; BORGES, ANA F. S.; XIMENES, VALDECIR F.; FREM, REGINA C. G.; LISBOA-FILHO, PAULO N. Encapsulation of collagenase within biomimetically mineralized metal-organic frameworks: designing biocomposites to prevent collagen degradation. NEW JOURNAL OF CHEMISTRY, v. 43, n. 2, p. 1017-1024, JAN 14 2019. Web of Science Citations: 0.

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