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Effect of protease inhibitors on collagen degradation potential from sound, sclerotic and caries-affected dentin

Grant number: 15/10566-7
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2015
Effective date (End): August 31, 2017
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Paulo Henrique dos Santos
Grantee:Bruna de Oliveira Reis
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The purpose of the study is to evaluate, through the tests of ultimate tensile strength and nanoindentation, the influence of proteases inhibitors on the collagen degradation from sound, sclerotic and caries-affected dentin. Thirty-nine human molars will be used, 13 for each dentin condition. Three slices will be obtained from each tooth. For the ultimate tensile strength, the teeth will be sectioned perpendicular to the long axis, worn in an hourglass shape and divided into 3 groups according to the protease inhibitor used: Group 1: artificial saliva (control group); Group 2: 2% chlorhexidine; Group 3: 0.5% EGCG (epigallocatechin gallate). After incubation, the samples will be subjected to enzymatic degradation (collagenase from Clostridium histolyticum), and then be submitted to the ultimate tensile strength test (MPa). The mechanical properties of dentin nanohardness and elastic modulus will be measured in digital nanoindenter under 1000¼N load. Data of ultimate tensile strength and mechanical properties of nanohardness and elastic modulus of collagen will be subjected to statistical analysis to verify the significance between the means (± = 0.05).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA-REIS, B.; MALULY-PRONI, A. T.; FAGUNDES, T. C.; VASCONCELOS, G.; BRESCIANI, E.; PRAKKI, A.; DOS SANTOS, P. H. Influence of protease inhibitors on the degradation of sound, sclerotic and caries-affected demineralized dentin. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 97, p. 1-6, SEP 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
REIS, Bruna de Oliveira. Influência de inibidores de proteases no potencial de degradação do colágeno proveniente da dentina sadia, esclerótica e afetada por cárie. 2017. 85 f. Master's Dissertation - Universidade Estadual Paulista (Unesp) Faculdade de Odontologia.

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