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| Author(s): |
Bruna de Oliveira Reis
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Araçatuba. 2017-08-29. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba |
| Defense date: | 2017-08-15 |
| Advisor: | Paulo Henrique dos Santos; Ticiane Cestari Fagundes |
| Abstract | |
Objetive: To evaluate the influence of proteases inhibitors on the collagen degradation from sound, sclerotic and caries-affected dentin. Materials and Methods: Thirty-nine human molars were used, thirteen for each dentin condition. Three slices were obtained from each tooth, each one immersed in different solutions: 1) artificial saliva; 2) 2% chlorhexidine; 3) 0.5% EGCG. After incubation in the solutions for 1h, samples were subjected to enzymatic degradation by collagenase derived from Clostridium histolyticum. Mechanical properties of nanohardness (HIT) and elastic modulus (Er) of the three types of dentin were measured before and after degradation, as well as the microtensile strength. Results of the microtensile strength and nanoindentation tests were submitted to ANOVA two and three factors for repeated measurements, and Tukey post-test (α = 0.05). Results: Higher values of tensile strength were found for sound dentin in control (40.30 ± 21.38 MPa) and 0.5% EGCG (30.05 ± 19.67 MPa) groups. Before degradation, higher values of HIT (0.237 ± 0.062 GPa) and Er (5.58 ± 1.75 GPa) were found for the 0.5% EGCG group in caries-affected dentin. After degradation, 2% chlorhexidine group had higher values of HIT and Er for sound (0.134 ± 0.020 GPa and 3.57 ± 0.40 GPa) and sclerotic (0.201 ± 0.048 GPa and 4.30 ± 0.56 GPa) dentin. Conclusions: The use of 2% chlorhexidine, mainly in sclerotic dentin, was shown to be more effective in promoting increase in the microtensile strength and mechanical properties, before and after the degradation. The 0.5% EGCG showed a better effect on mechanical properties in caries-affected dentin, before the enzymatic degradation. Clinical Relevance: The effective knowledge of the mechanism of action of protease inhibitors in different types of dentin could contribute to the improvement of the resistance of the substrate and to the longer longevity of the bonding processes on this tissue. (AU) | |
| FAPESP's process: | 15/10566-7 - Effect of protease inhibitors on collagen degradation potential from sound, sclerotic and caries-affected dentin |
| Grantee: | Bruna de Oliveira Reis |
| Support Opportunities: | Scholarships in Brazil - Master |