Dentin degradation and study of biomodifying catechols

• Grant number: 19/20576-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2021
• End date: January 31, 2024
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Flávio Henrique Baggio Aguiar
• Grantee: Flávio Henrique Baggio Aguiar
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Alberto Martín Rivera Dávila ; Ana Luiza de Mattos Guaraldi ; Dr Klaus Rischka ; Rodrigo Jardim Monteiro da Fonseca ; Ubirajara Pereira Rodrigues Filho

Abstract

Host metalloproteinases play a significant role in the degradation of the adhesive interface. One strategy to decrease the effects of MMPs on the dentin substrate is to inactivate the active collagen sites to which these enzymes bind. Preliminary results obtained by our group suggest that the biomodification of the dentin substrate using catechols is a good alternative to inactivate the active sites of MMPs, avoiding the degradation and denaturation of collagen. Continuing the studies carried out, the objective of this project is: (1) to investigate the differential expression of MMP transcripts, their tissue inhibitors and bacterial proteases, using young and aged teeth metatranscriptomes and dentin substrates of carious lesions and endodontically treated teeth, in order to identify profiles according to the patient's age and according to the condition of the dental element; and (2) to study the degradation and adhesive infiltration in substrates treated with natural biomodifiers (Dopa, dopamine and caffeic acid), under different dentin surface conditioners. Data will be analyzed according to their distribution (normality and homoscedasticity analysis), in addition to bioinformatics tools for the treatment and analysis of transcriptomes. Expected results aim to understand the role of these proteases (endogenous and exogenous) and their interaction with tissue inhibitors in the degradation of the adhesive interface, in addition to developing a methodology capable of obtaining transcripts from dentin substrate, as well as a new technique that minimizes the action of MMPs in the degradation of the hybrid layer, with the use of biomodifying materials. (AU)